Methylstat, jumonji C domain-containing histone demethylase inhibitor (ab144566)
Key features and details
- Selective Jumonji C domain-containing histone demethylase inhibitor
- CAS Number: 1310877-95-2
- Purity: > 90%
- Soluble in DMSO to 100 mM
- Form / State: Solid
- Source: Synthetic
Overview
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Product name
Methylstat, jumonji C domain-containing histone demethylase inhibitor -
Description
Selective Jumonji C domain-containing histone demethylase inhibitor -
Biological description
Selective Jumonji C domain-containing histone demethylase inhibitor. Anticancer agent, inhibits esophageal carcinoma cell growth (GI50 = 5.1 μM). Cell-permeable. Active in vivo and in vitro.
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Purity
> 90% -
CAS Number
1310877-95-2 -
Chemical structure
Properties
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Chemical name
(2E)-4-[Hydroxy[4-[[[4-[[[(1-naphthalenylamino)carbonyl]oxy]methyl]phenyl]methyl]amino]butyl]amino]-4-oxo-2-butenoic acid methyl ester -
Molecular weight
505.57 -
Molecular formula
C28H31N3O6 -
PubChem identifier
53392493 -
Storage instructions
Store at +4°C. It is important to note that this product is reported to be light sensitive. Store under desiccating conditions. The product can be stored for up to 12 months. -
Solubility overview
Soluble in DMSO to 100 mM -
Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
COC(=O)/C=C/C(=O)N(CCCCNCC1=CC=C(C=C1)COC(=O)NC2=CC=CC3=CC=CC=C32)O -
Source
Synthetic
Images
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Functional Studies - Methylstat, jumonji C domain-containing histone demethylase inhibitor (ab144566)ab9050 staining Histone H3 (tri methyl K36) in MCF-7 cells. The cells were incubated with 15µM Methylstat (ab144566) for 24 hours (Treated) or solvent-only for control purposes (Non-treated). Cells were fixed with 100% methanol (5min) and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab9050 at 0.1µg/ml and ab195889 Mouse monoclonal [DM1A] to alpha Tubulin - Alexa Fluor® 594 at 2µg/ml (shown in pseudo colour red) overnight at +4°C, followed by a further incubation at room temperature for 1h with an AlexaFluor®488 Goat anti-Rabbit secondary (ab150081) at 1/1000 dilution (shown in green). Nuclear DNA was labelled in blue with DAPI. Negative controls: 1– anti-rabbit secondary only (solvent only); 2 – anti-rabbit secondary only (Treated). The secondary antibody control demonstrates that the labelling observed is due only to the binding of the secondary antibody to the primary antibody