Methionine Assay Kit (Fluorometric) (ab234041)
Key features and details
- Assay type: Quantitative
- Detection method: Fluorescent
- Platform: Microplate reader
- Sample type: Cell Lysate, Plasma, Serum
Overview
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Product name
Methionine Assay Kit (Fluorometric) -
Detection method
Fluorescent -
Sample type
Serum, Plasma, Cell Lysate -
Assay type
Quantitative -
Product overview
Methionine Assay Kit (Fluorometric) (ab234041) utilizes an enzymatic mechanism by which metabolism of methionine is coupled with the stoichiometric generation of hydrogen peroxide. A probe generates a fluorometric signal that can be quantified at Ex/Em: 535/587 nm. The assay shows greater than 10-fold specificity for methionine over cysteine and greater than 40-fold selectivity over other standard and non-standard amino acids. After utilization of a proprietary Sample Clean-Up Mix, the method is suitable for use in blood samples, and can detect concentrations as low as 0.5 µM, or 25 pmole Methionine.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Methionine Assay Buffer 1 x 25ml Methionine Buffer Supplement 1 vial Methionine Developer 1 x 200µl Methionine Enzyme Mix I 1 x 200µl Methionine Enzyme Mix II 1 vial Methionine Probe 1 x 200µl Methionine Standard (100 mM) 1 x 100µl Sample Clean-up Mix 1 vial -
Research areas
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Relevance
Methionine is an essential amino acid, it cannot be synthesized in humans. However, in plants and microorganisms, methionine is synthesized from aspartic acid and cysteine. Methionine plays a role in cysteine, carnitine and taurine synthesis by the transsulfuration pathway, lecithin production, the synthesis of phosphatidylcholine and other phospholipids. Improper conversion of methionine can lead to atherosclerosis. Methionine is a chelating agent. -
Alternative names
- Met
- Methionine
Images
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Example Standard Curve
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Methionine in human plasma.The assay was run on various volumes of treated plasma and the results averaged. Methionine concentration was determined to be 18.6 µM in human plasma (2-12 µL; undiluted).
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Specificity of the assayThe assay was run in the presence of 1 nmole (1000 pmole) each of the amino acids Methionine, Cysteine, Homocysteine and Alanine. Numbers are relative to 100% response to methionine.