Mercury Assay Kit (ab233467)
Key features and details
- Detection method: Fluorescent
- Platform: Microplate reader
Overview
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Product name
Mercury Assay Kit -
Detection method
Fluorescent -
Product overview
Mercury Assay Kit (ab233467) offers a fluorescence-based assay for measuring mercury ion (Hg2+) with high selectivity. Mercury sensor 590 itself is nearly non-fluorescent, but generates more than 500-fold fluorescence enhancement upon binding Hg2+ ion. The fluorescence signal can be measured with a fluorescence microplate reader at Ex/Em= 540/590 nm. With this kit, we were able to detect as low as 8 µM Hg2+ in a 100 µL reaction volume.
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Notes
Mercury ion is considered to be one of the most hazardous pollutants and highly dangerous materials. The high toxicity of Hg2+ is caused by its high affinity to the thiol groups in biological ligands such as proteins, DNA, and enzymes. When Hg2+ is absorbed in the human body from the environment, it induces aberrations in microtubules, ion channels, and mitochondria presumably and significant damage to the kidneys, heart, brain, stomach, intestines, central nervous system and immune systems. In addition, mercury accumulates through food chains or atmosphere in the ecological system, and has a relatively long atmospheric residence time because of its non-biodegradation. Therefore, highly selective and sensitive detection of mercury is of toxicological and environmental importance.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Assay Buffer 1 x 10ml DMSO 1 x 100µl Mercury reagent 1 vial
Images
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Hg2+ was measured with ab233467 in a 96-well solid black plate using a Gemini microplate reader (Molecular Devices).
As low as 8 µM (A) or 1.6 ppm (B) mercury (II) perchlorate was detected with 30 minutes incubation.