Maltose Phosphorylase Activity Kit (Fluorometric) (ab273317)
Key features and details
- Assay type: Enzyme activity (quantitative)
- Detection method: Colorimetric/Fluorometric
- Platform: Microplate
- Sample type: Cell Lysate
Overview
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Product name
Maltose Phosphorylase Activity Kit (Fluorometric) -
Detection method
Colorimetric/Fluorometric -
Sample type
Cell Lysate -
Assay type
Enzyme activity (quantitative) -
Assay duration
Multiple steps standard assay -
Product overview
Maltose Phosphorylase Assay Kit (Fluorometric) (ab273317) is the first commercial assay to determine the Maltose Phosphorylase activity in different bacterial lysates. The assay converts maltose to produce Glucose, which is then detected by a set of enzymatic reactions to generate a fluorescent product with a Ex/Em 535/587 nm. The fluorescence signal is directly proportional to the Maltose Phosphorylase activity.
This Kit is rapid, sensitive and a convenient tool for detecting Maltose Phosphorylase activity. It can detect as low as 0.5 mU under the assay conditions.
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Tested applications
Suitable for: Othermore details -
Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Glucose Standard 1 x 100µl Maltose (Lyophilized) 1 vial Maltose Assay Buffer 1 x 25ml Maltose Developer (Lyophilized) 1 vial Maltose Phosphorylase 1 x 50µl Maltose Probe 1 x 200µl -
Research areas
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Relevance
Maltose phosphorylase is a dimeric enzyme that catalyzes the conversion of maltose and inorganic phosphate into beta D glucose 1 phosphate and glucose without requiring any cofactors, such as pyridoxal phosphate. The enzyme is part of operons that are involved in maltose/malto oligosaccharide metabolism. -
Alternative names
- Maltose phosphorylase
- mapA
- mpA
Images
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Example data
Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
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Example dataReaction curve for Maltose Phosphorylase activity.
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Example dataMaltose Phosphorylase activity using E. coli and Staphylococcus aureus cell lysates.