Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ENO1 + ENO2 + ENO3 antibody [EPR18407] - BSA and Azide free (ab250997)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18407] to ENO1 + ENO2 + ENO3 - BSA and Azide free
  • Suitable for: Flow Cyt, ICC, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-ENO1 + ENO2 + ENO3 antibody [EPR18407] - BSA and Azide free
    See all ENO1 + ENO2 + ENO3 primary antibodies

  • Description

    Rabbit monoclonal [EPR18407] to ENO1 + ENO2 + ENO3 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, ICC, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human ENO1 + ENO2 + ENO3 aa 250-350. The exact sequence is proprietary.
    Database link: P13929

  • General notes

    Ab250997 is the carrier-free version of ab189891. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab250997 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Images

  • Western blot - Anti-ENO1 + ENO2 + ENO3 antibody [EPR18407] - BSA and Azide free (ab250997)
    Western blot - Anti-ENO1 + ENO2 + ENO3 antibody [EPR18407] - BSA and Azide free (ab250997)
    All lanes : Anti-ENO1 + ENO2 + ENO3 antibody [EPR18407] (ab189891) at 1/1000 dilution

    Lane 1 : Human ENO1 full length recombinant protein
    Lane 2 : Human ENO2 full length recombinant protein
    Lane 3 : Human ENO3 full length recombinant protein

    Lysates/proteins at 0.02 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 47 kDa
    Observed band size: 47 kDa


    Exposure time: 5 seconds


    This data was developed using ab189891, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Human ENO1 full length recombinant protein (ab89248) contains aa1-434. Human ENO2 full length recombinant protein contains aa1-434 with a His-Tag®. Human ENO3 full length recombinant protein (ab113127) contains aa1-434 with a His-Tag®. Human ENO2 full length recombinant protein was made in-house.

  • Western blot - Anti-ENO1 + ENO2 + ENO3 antibody [EPR18407] - BSA and Azide free (ab250997)
    Western blot - Anti-ENO1 + ENO2 + ENO3 antibody [EPR18407] - BSA and Azide free (ab250997)
    All lanes : Anti-ENO1 + ENO2 + ENO3 antibody [EPR18407] (ab189891) at 1/1000 dilution

    Lane 1 : Human fetal heart lysate
    Lane 2 : Human fetal kidney lysate
    Lane 3 : Human fetal spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 47 kDa
    Observed band size: 47 kDa



    This data was developed using ab189891, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times:  Lanes 1-2: 5 seconds; Lane 3: 15 seconds.

  • Western blot - Anti-ENO1 + ENO2 + ENO3 antibody [EPR18407] - BSA and Azide free (ab250997)
    Western blot - Anti-ENO1 + ENO2 + ENO3 antibody [EPR18407] - BSA and Azide free (ab250997)
    All lanes : Anti-ENO1 + ENO2 + ENO3 antibody [EPR18407] (ab189891) at 1/5000 dilution

    Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 3 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
    Lane 4 : A431 (Human epidermoid carcinoma cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 47 kDa
    Observed band size: 47 kDa


    Exposure time: 3 seconds


    This data was developed using ab189891, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-ENO1 + ENO2 + ENO3 antibody [EPR18407] - BSA and Azide free (ab250997)
    Western blot - Anti-ENO1 + ENO2 + ENO3 antibody [EPR18407] - BSA and Azide free (ab250997)
    Lanes 1-4 : Anti-ENO1 + ENO2 + ENO3 antibody [EPR18407] (ab189891) at 1/1000 dilution
    Lanes 5-7 : Anti-ENO1 + ENO2 + ENO3 antibody [EPR18407] (ab189891) at 1/5000 dilution

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Mouse heart tissue lysate
    Lane 3 : Rat brain tissue lysate
    Lane 4 : Rat heart tissue lysate
    Lane 5 : C6 (Rat glial tumor cell line) whole cell lysate
    Lane 6 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 7 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Predicted band size: 47 kDa
    Observed band size: 47 kDa



    This data was developed using ab189891, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: Lanes 1-4: 5 seconds; Lanes 5-7: 1 second.

  • Immunocytochemistry - Anti-ENO1 + ENO2 + ENO3 antibody [EPR18407] - BSA and Azide free (ab250997)
    Immunocytochemistry - Anti-ENO1 + ENO2 + ENO3 antibody [EPR18407] - BSA and Azide free (ab250997)
    This data was developed using ab189891, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling ENO1 + ENO2 + ENO3 with ab189891 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing mostly cytoplasmic staining on Hela cells. The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin - Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG (AlexaFluor®594) preadsorbed (ab150120) at 1/1000 dilution (red). The negative controls are as follows:- -ve control 1: ab189891 at 1/500 dilution followed by ab150120 at 1/1000 dilution. -ve control 2: ab7291 at 1/1000 dilution followed by ab150077 at 1/1000 dilution.
  • Immunocytochemistry - Anti-ENO1 + ENO2 + ENO3 antibody [EPR18407] - BSA and Azide free (ab250997)
    Immunocytochemistry - Anti-ENO1 + ENO2 + ENO3 antibody [EPR18407] - BSA and Azide free (ab250997)
    This data was developed using ab189891, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (Mouse embryonic fibroblast cell line) cells labeling ENO1 + ENO2 + ENO3 with ab189891 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing mostly cytoplasmic staining on NIH/3T3 cells. The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody -Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG (AlexaFluor®594) preadsorbed (ab150120) at 1/1000 dilution (red). The negative controls are as follows:- -ve control 1: ab189891 at 1/500 dilution followed by ab150120 at 1/1000 dilution. -ve control 2: ab7291 at 1/1000 dilution followed by ab150077 at 1/1000 dilution.
  • Flow Cytometry - Anti-ENO1 + ENO2 + ENO3 antibody [EPR18407] - BSA and Azide free (ab250997)
    Flow Cytometry - Anti-ENO1 + ENO2 + ENO3 antibody [EPR18407] - BSA and Azide free (ab250997)
    This data was developed using ab189891, the same antibody clone in a different buffer formulation.Flow cytometric analysis of 4% paraformaldehyde-fixed NIH/3T3 (Mouse embryonic fibroblast cell line) labeling ENO1 + ENO2 + ENO3 with ab189891 at 1/70 dilution (red) compared with a Rabbit IgG,monoclonal - Isotype control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti Rabbit IgG (Alexa Fluor® 488) at 1/500 dilution was used as the secondary antibody.
  • Anti-ENO1 + ENO2 + ENO3 antibody [EPR18407] - BSA and Azide free (ab250997)
    Anti-ENO1 + ENO2 + ENO3 antibody [EPR18407] - BSA and Azide free (ab250997)

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