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Anti-RSF1 antibody [EPR3749(2)] - BSA and Azide free (ab232564)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3749(2)] to RSF1 - BSA and Azide free
  • Suitable for: Flow Cyt, ICC/IF, WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-RSF1 antibody [EPR3749(2)] - BSA and Azide free
    See all RSF1 primary antibodies

  • Description

    Rabbit monoclonal [EPR3749(2)] to RSF1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, ICC/IF, WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human colon tissue.

  • General notes

    Ab232564 is the carrier-free version of ab109002. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab232564 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Flow Cytometry - Anti-RSF1 antibody [EPR3749(2)] - BSA and Azide free (ab232564)
    Flow Cytometry - Anti-RSF1 antibody [EPR3749(2)] - BSA and Azide free (ab232564)
    Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling RSF1 with purified ab109002 at 1:80 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109002).

  • Immunocytochemistry/ Immunofluorescence - Anti-RSF1 antibody [EPR3749(2)] - BSA and Azide free (ab232564)
    Immunocytochemistry/ Immunofluorescence - Anti-RSF1 antibody [EPR3749(2)] - BSA and Azide free (ab232564)

    Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling RSF1 with Purified ab109002 at 1:100 (8.7 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 μg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109002).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RSF1 antibody [EPR3749(2)] - BSA and Azide free (ab232564)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RSF1 antibody [EPR3749(2)] - BSA and Azide free (ab232564)

    Paraffin-embedded human endometrial adenocarcinoma tissue stained for RSF1 with ab109002 (unpurified) at a 1/250 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109002).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RSF1 antibody [EPR3749(2)] - BSA and Azide free (ab232564)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RSF1 antibody [EPR3749(2)] - BSA and Azide free (ab232564)

    Paraffin-embedded human tonsil tissue stained for RSF1 with ab109002 (unpurified) at a 1/250 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109002).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RSF1 antibody [EPR3749(2)] - BSA and Azide free (ab232564)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RSF1 antibody [EPR3749(2)] - BSA and Azide free (ab232564)

    Paraffin-embedded human colon tissue stained for RSF1 with ab109002 (unpurified) at a 1/250 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109002).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-RSF1 antibody [EPR3749(2)] - BSA and Azide free (ab232564)
    Anti-RSF1 antibody [EPR3749(2)] - BSA and Azide free (ab232564)

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