Maltose and Glucose Assay Kit (ab65335)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric/Fluorometric
- Platform: Microplate reader
- Assay time: 1 hr
- Sample type: Cell culture supernatant, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
- Sensitivity: 0.01 nmol/well
Overview
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Product name
Maltose and Glucose Assay Kit -
Detection method
Colorimetric/Fluorometric -
Sample type
Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts -
Assay type
Quantitative -
Sensitivity
> 0.01 nmol/well -
Range
0.01 nmol/well - 10 nmol/well -
Assay time
1h 00m -
Product overview
Maltose and Glucose Assay Kit (ab65335) can measure glucose or maltose levels in a variety of biological samples (e.g. serum, plasma, body fluids, food, growth medium, etc.). The assay is based on the ability of glucose oxidase to specifically oxidize free glucose, producing a product that interacts with the glucose probe. The amount of product can be either colorimetric (spectrophotometry at OD= 570 nm) or fluorometric (Ex/Em = 535/587 nm) method.
This kit can detect 10 pmol to 10 nmol glucose per assay. It can also be used for isomaltose detection.
Visit our FAQs page for tips and troubleshooting. -
Notes
Glucose (C6H12O6; FW: 180.16) and Maltose (C12H22O11; FW: 342.3) are the main fuel sources to generate the universal energy molecule ATP. Maltose is the major disaccharide that generated from hydrolysis of starch in food. Maltose contains two glucose units joined by a α-1,4-glycosidic linkage, which can be easily converted to two glucoses by α-D-glucosidase.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 100 tests alpha-D-Glucosidase (Lyophilized) Blue 1 vial Assay Buffer WM 1 x 25ml Glucose Enzyme Mix (lyophilized) Green 1 vial Glucose Probe Red 1 x 0.2ml Maltose Standard (100 nmol/µl) Yellow 1 x 100µl -
Research areas
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Relevance
Maltose (C12H22O11; fW:342.30) is a disaccharide formed from two units of glucose (C6H12O6; FW: 180.16) joined via a a-1,4-glycosidic linkage. Maltose is the major disaccharide generated from hydrolysis of starch and in living organisms, maltose is hydrolyzed by maltase. Once inside the cells, glucose will be processed and ener the glycolysis pathway to ultimately produce enery in form of ATP.
Images
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Functional Studies - Maltose and Glucose Assay Kit (ab65335)Maltose Standard Curve. Assays were performed using various amounts of Maltose standard according to kit instructions. Circle is maltose with a-D-Glucosidase. Square is maltose without a-D-Glucosidase. Triangle is glucose with 2 times nmol of Maltose.