Anti-IL-11 antibody (ab76589)
Key features and details
- Rabbit polyclonal to IL-11
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-IL-11 antibody
See all IL-11 primary antibodies -
Description
Rabbit polyclonal to IL-11 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human IL-11 aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available asab90835) -
Positive control
- Purchase matching WB positive control:Recombinant human IL-11 protein
- This antibody gave a positive signal in Human Plasma Total Protein.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab76589 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications Macaque monkeyApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 27 kDa (predicted molecular weight: 21 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 27 kDa (predicted molecular weight: 21 kDa).Target
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Function
Directly stimulates the proliferation of hematopoietic stem cells and megakaryocyte progenitor cells and induces megakaryocyte maturation resulting in increased platelet production. -
Sequence similarities
Belongs to the IL-6 superfamily. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 3589 Human
- Omim: 147681 Human
- SwissProt: P20809 Human
- Unigene: 467304 Human
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Alternative names
- Adipogenesis inhibitory factor antibody
- AGIF antibody
- IL 11 antibody
see all
Images
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Western blot - Anti-IL-11 antibody (ab76589)Anti-IL-11 antibody (ab76589) at 1 µg/ml + Human Plasma Total Protein Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 21 kDa
Observed band size: 27 kDa why is the actual band size different from the predicted?
Additional bands at: 105 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 15 minutesThe 27 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to IL-11.
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Western blot - Anti-IL-11 antibody (ab76589)
Datasheets and documents
References (2)
ab76589 has been referenced in 2 publications.
- Marthandan S et al. Similarities in Gene Expression Profiles during In Vitro Aging of Primary Human Embryonic Lung and Foreskin Fibroblasts. Biomed Res Int 2015:731938 (2015). WB . PubMed: 26339636
- Jiang Y et al. Downregulation of SPARC expression inhibits the invasion of human trophoblast cells in vitro. PLoS One 8:e69079 (2013). WB . PubMed: 23935929
Images
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Western blot - Anti-IL-11 antibody (ab76589)Anti-IL-11 antibody (ab76589) at 1 µg/ml + Human Plasma Total Protein Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 21 kDa
Observed band size: 27 kDa why is the actual band size different from the predicted?
Additional bands at: 105 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 15 minutesThe 27 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to IL-11.
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Western blot - Anti-IL-11 antibody (ab76589)
