Malate Dehydrogenase Activity Assay Kit (Fluorometric) (ab235593)
Key features and details
- Assay type: Cell-based (quantitative)
- Detection method: Fluorescent
- Platform: Microplate reader
- Sample type: Adherent cells, Suspension cells, Tissue
- Sensitivity: 40 µU
Overview
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Product name
Malate Dehydrogenase Activity Assay Kit (Fluorometric) -
Detection method
Fluorescent -
Sample type
Tissue, Adherent cells, Suspension cells -
Assay type
Cell-based (quantitative) -
Sensitivity
40 µU -
Species reactivity
Predicted to work with: Mammals
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Product overview
The Malate Dehydrogenase Activity Assay Kit (ab235593) is a quick and easy method for monitoring Malate Dehydrogenase (MDH) activity in wide variety of samples. In this assay, MDH converts malate into intermediate products, which will further react with the Probe forming a fluorophore (Ex/Em=535/587nm). The kit is simple, sensitive and high-throughput adaptable and can detect as low as 40 μU of MDH activity.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests MDH Assay Buffer 1 x 25ml MDH Developer 1 vial MDH Positive Control 1 vial MDH Probe 1 x 450µl MDH Substrate 1 vial NADH Standard 1 vial -
Research areas
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Function
Catalyzes the reversible oxidation of malate to oxaloacetate. -
Sequence similarities
Belongs to the LDH/MDH superfamily. MDH type 1 family. - Information by UniProt
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Alternative names
- Malate dehydrogenase
- mdh
- MDH_ECOLI
Images
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Example Data
NADH standard curve.
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Example DataMalate Dehydrogenase activities in rat muscle extract (0.011 μg protein), Jurkat cell lysate (0.026 μg protein), Saccharomyces mitochondria (0.05 μg) and MDH positive control
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Example DataSpecific MDH activities in rat muscle extract, Jurkat cell lysate and yeast mitochondria.
