Magnesium Assay Kit (ab272535)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate
- Sample type: Food samples, Milk, Serum, Urine
Overview
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Product name
Magnesium Assay Kit
See all Magnesium kits -
Detection method
Colorimetric -
Sample type
Milk, Urine, Serum, Food samples -
Assay type
Quantitative -
Range
41 µM - 1200 µM -
Product overview
Magnesium Assay Kit (ab272535) is a simple, direct and automation-ready procedures for measuring magnesium concentration in biological samples. This assay kit is designed to measure magnesium directly in biological samples without any pretreatment. A calmagite dye in the kit forms a colored complex specifically with magnesium. The intensity of the color, measured at 500 nm, is directly proportional to the magnesium concentration in the sample. The optimized formulation minimizes interference by potential substances.
Sensitive and accurate Use as little as 5 μL sample. Linear detection range 0.1 mg/dL (41μM) to 3 mg/dL (1.2 mM) Mg2+ in 96-well plate assay.
Simple and high-throughput: The procedure involves addition of two reagents and measuring OD500nm. Can be readily automated as a highthroughput assay for thousands of samples per day.
Improved reagent stability and versatility: The optimized formulation has greatly enhanced reagent and signal stability. Cuvette or 96-well plate assay.
Low interference in biological samples: Assays can be directly performed in serum and urine samples.
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Tested applications
Suitable for: Functional Studiesmore details -
Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 250 tests EDTA (0.1 M) 2 x 1.5ml Reagent A 1 x 25ml Reagent B 1 x 25ml Standard (10mg/dL Mg) 1 x 1ml -
Research areas
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Relevance
Magnesium is the 11th most abundant element by mass in the human body. Magnesium is essential to all living cells where it plays an important role in facilitating the processing of biological polyphosphates like ATP, DNA, RNA and enzyme functions. Magnesium is the metallic ion at the center of chlorophyll, and a common additive to fertilizers. Magnesium compounds are used as laxatives, antacids, and used to stabilize abnormal nerve excitation and blood vessel spasm i.e., eclampsia. -
Alternative names
- Mg++
- Mg+2
- Mg2+
Images
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Magnesium Assay Kit Standard Curve
Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.