LVV Hemorphin 7 ELISA kit (ab136942)
Key features and details
- Sensitivity: 6.1 pg/ml
- Range: 9.8 pg/ml - 10000 pg/ml
- Sample type: Serum, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Competitive
- Reacts with: Rat, Human
Overview
-
Product name
LVV Hemorphin 7 ELISA kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% LVV Hem 7 20 708.1pg/ml 4.1% LVV Hem 7 20 305.5pg/ml 8.8% LVV Hem 7 20 71.5pg/ml 20.9% Inter-assay Sample n Mean SD CV% LVV Hem 7 707pg/ml 9.2% LVV Hem 7 334.1pg/ml 9.5% LVV Hem 7 72.3pg/ml 16.5% -
Sample type
Serum, Tissue Extracts -
Assay type
Competitive -
Sensitivity
6.1 pg/ml -
Range
9.8 pg/ml - 10000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum = 94 100ng/ml - 5000ng/ml Tissue Extracts = 95 100ng/ml - 5000ng/ml -
Assay time
3h 0m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Rat, Human -
Product overview
Abcam’s LVV Hemorphin 7 in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of LVV Hemorphin 7 in serum and tissue homogenates. This kit it not intended for use with plasma samples.
A polyclonal anti-Rabbit-IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with a solution of LVV Hemorphin 7 conjugated to biotin, followed by a solution of polyclonal antibody to LVV Hemorphin 7. The plate is washed to remove unbound reagents. A solution of streptavidin-HRP conjugate is then added. After further incubation the excess reagents are washed away and TMB substrate is added, which is catalyzed by HRP to generate a yellow color. A stop solution changes this color from yellow to blue, and the intensity of this blue coloration is inversely proportional to the amount of LVV Hemorphin 7 captured in the plate.
-
Notes
Analyte Sequence Percent cross reactivities in the range of 0.1 pM - 500 nM LVV Hemorphin 7 LVVYPWTQRF 100 Leu-Valorphin-Arg (LVV Hemorphin-6) VVYPWTQR Valorphin VVYPWTQ Ang(1-12) DRVYIHPFHLVI Ang I DRVYIHPFHL Ang(1-9) DRVYIHPFH Ang II DRVYIHPF Ang(1-7) DRVYIHP Ang A ARVYIHPF Ang III RVYIHPF Ang IV VYIHPF -
Platform
Microplate
Properties
-
Storage instructions
Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 27ml Assay Buffer 16 1 x 30ml Goat anti-rabbit IgG Microplate (12 x 8 wells) 1 x 96 tests HRP- Streptavidin Conjugate 1 x 12.5µg LVV Hemorphin 7 Antibody 1 x 6ml LVV Hemorphin 7 Conjugate 1 x 6ml LVV Hemorphin 7 Standard 2 x 40ng Plate Sealer 2 units Stop Solution 2 1 x 10ml TMB Substrate 2 x 10ml -
Research areas
-
Function
Involved in oxygen transport from the lung to the various peripheral tissues.
LVV-hemorphin-7 potentiates the activity of bradykinin, causing a decrease in blood pressure.
Spinorphin: functions as an endogenous inhibitor of enkephalin-degrading enzymes such as DPP3, and as a selective antagonist of the P2RX3 receptor which is involved in pain signaling, these properties implicate it as a regulator of pain and inflammation. -
Tissue specificity
Red blood cells. -
Involvement in disease
Heinz body anemias
Beta-thalassemia
Sickle cell anemia
Beta-thalassemia, dominant, inclusion body type -
Sequence similarities
Belongs to the globin family. -
Post-translational
modificationsGlucose reacts non-enzymatically with the N-terminus of the beta chain to form a stable ketoamine linkage. This takes place slowly and continuously throughout the 120-day life span of the red blood cell. The rate of glycation is increased in patients with diabetes mellitus.
S-nitrosylated; a nitric oxide group is first bound to Fe(2+) and then transferred to Cys-94 to allow capture of O(2).
Acetylated on Lys-60, Lys-83 and Lys-145 upon aspirin exposure. PubMed:16916647 reports the identification of HBB acetylated on Lys-145 in the cytosolic fraction of HeLa cells. This may have resulted from contamination of the sample. - Information by UniProt
-
Alternative names
- Beta-globin
- HBB
- HBB_HUMAN
see all -
Database links
- Entrez Gene: 3043 Human
- Entrez Gene: 24440 Rat
- SwissProt: P68871 Human
- SwissProt: P69905 Human
- Unigene: 523443 Human
Images
-
Typical Standard Curve
Representative Standard Curve using ab136942.
-
Typical Parallelism GraphDose‐response curves from human plasma and serum diluted into assay buffer were compared to the LVV Hemorphin 7 standard curve. The parallel response indicates the standard effectively mimics the native protein.
