Live Cell Luciferase Assay Kit (ab253393)
Overview
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Product name
Live Cell Luciferase Assay Kit -
Product overview
This kit contains the all of the reagents including the primary substrate of the firefly luciferase light producing system, D-luciferin, as well as the lysis and luciferase enzyme assay buffers.
Firefly luciferase (EC 1.13.12.7) is the enzyme used in chemiluminescent reactions, producing light by the oxidation of D-luciferin with oxygen and adenosine triphosphate (ATP). Luciferase is used in numerous biochemical assays, where the gene is either cloned into cells or tissues as a reporter or marker gene. The Live Cell Luciferase Assay Kit kit will perform 1000 luciferase assays using a microtiterplate format (100 µL reaction volume, 0.1 mM final reaction concentration). The synthetic beetle luciferin substrate contained in the kit is also available separately as a lyophilized powder, D-Luciferin.
This kit is sold by Marker Gene under product code M0626. -
Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Properties
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Storage instructions
Please refer to protocols. -
Components 1 units Cell Lysis Buffer 1 x 10ml D-Luciferin with ATP Reagent 1 vial Reaction Buffer 1 x 10ml