Lipolysis Assay Kit (Fluorometric) (ab185434)
Key features and details
- Assay type: Quantitative
- Detection method: Fluorescent
- Platform: Microplate reader
- Sample type: Cell culture supernatant
- Sensitivity: 0.02 nmol/well
Overview
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Product name
Lipolysis Assay Kit (Fluorometric)
See all Lipolysis kits -
Detection method
Fluorescent -
Sample type
Cell culture supernatant -
Assay type
Quantitative -
Sensitivity
Species reactivity
Reacts with: Mammals, Other speciesProduct overview
Lipolysis Assay Kit (Fluorometric) ab185434 is simple, easy-to-use and highly sensitive.
This lipolysis assay measures glycerol released from 3T3-L1 cells as early as 1 hour after induction of lipolysis. It is suitable for measuring trace amounts of glycerol from samples. In the assay, glycerol reacts with Glycerol Enzyme Mix to form an intermediate that in turn reacts with Glycerol Developer and PicoProbe™ to generate the fluorescence product. The fluorescence intensity is directly proportional to the amount of glycerol.
The lipolysis assay kit can detect less than 20 pmol of glycerol.
Notes
Lipolysis is the hydrolysis of triglycerides within the cell into glycerol and free fatty acids. The glycerol and free fatty acids are then released into the bloodstream or culture media. Lipolysis occurs in essentially all cells, but is most abundant in white and brown adipose tissue.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.Platform
Microplate readerProperties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 100 tests Glycerol Assay Buffer WM 1 x 25ml Glycerol Developer 1 vial Glycerol Enzyme Mix Green 1 vial Glycerol Standard (100 mM) Yellow 1 x 0.2ml Isoproterenol (10mM) 1 x 50µl Lipolysis Assay Buffer 1 x 17ml Lipolysis Wash Buffer 1 x 22ml PicoProbe Blue 1 x 0.4ml -
Research areas
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 100 tests Glycerol Assay Buffer WM 1 x 25ml Glycerol Developer 1 vial Glycerol Enzyme Mix Green 1 vial Glycerol Standard (100 mM) Yellow 1 x 0.2ml Isoproterenol (10mM) 1 x 50µl Lipolysis Assay Buffer 1 x 17ml Lipolysis Wash Buffer 1 x 22ml PicoProbe Blue 1 x 0.4ml -
Research areas
Images
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Glycerol Standard Curve.
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Measurement of glycerol levels in media of 3T3-L1 cells.5 μl of 3T3-L1 cells treated with vehicle control (H2O) or 100 nM Isoproterenol for 0-3 hours. Measurements for 0 hours were undetectable.
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Inhibition of Isoproterenol stimulated lipolysis.Inhibition of Isoproterenol (100 nM) stimulated lipolysis by treatment with 100 nM insulin (measured using 5 μl of media of 3T3-L1 cells). Control was vehicle (H2O) treated cells.
