L-Amino Acid Assay Kit (ab65347)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric/Fluorometric
- Platform: Microplate reader
- Assay time: 30 min
- Sample type: Cell culture supernatant, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
- Sensitivity: 40 µM
Overview
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Product name
L-Amino Acid Assay Kit -
Detection method
Colorimetric/Fluorometric -
Sample type
Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts -
Assay type
Quantitative -
Sensitivity
Assay time
0h 30mProduct overview
L-Amino Acid Assay Kit ab65347 is used measure the level of L-amino acids in biological samples. There is no requirement for sample pretreatment or purification.
The L-amino acid(s) level can be quantified using fluorometric (at Ex/Em = 535/587 nm) or colorimetric (at λ = 570 nm) methods in 96-well plates.
Amino acid assay protocol summary:
- add reaction mix to sample and standard wells
- incubate for 30 min
- analyze with microplate readerNotes
L-Amino acids are the most essential elements in biology. Accurately quantitating L-amino acids in body fluids or purified samples may provide valuable information for diagnostic or basic research studies.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.Platform
Microplate readerProperties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 100 tests L-Amino Acid Assay Buffer WM 1 x 25ml L-Amino Acid Enzyme Mix Green 1 vial L-amino Acid Probe Red 1 x 0.2ml L-Amino Acid Standard (4 nmol/µl) Yellow 1 x 300µl -
Research areas
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Relevance
Amino acids perform various important functions for the body and serve as the materials for the body's cells, hormones, and enzymes. Of the standard amino acids, all by glycine can exist in either of the two optical isomers, called D and L enantiomers, which are mirror images of each other. The L-amino acids represent the vast majority of amino acids found in proteins during translation in the ribosome, and are therefore the most essential element in biology and metabolic functions.
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 100 tests L-Amino Acid Assay Buffer WM 1 x 25ml L-Amino Acid Enzyme Mix Green 1 vial L-amino Acid Probe Red 1 x 0.2ml L-Amino Acid Standard (4 nmol/µl) Yellow 1 x 300µl -
Research areas
-
Relevance
Amino acids perform various important functions for the body and serve as the materials for the body's cells, hormones, and enzymes. Of the standard amino acids, all by glycine can exist in either of the two optical isomers, called D and L enantiomers, which are mirror images of each other. The L-amino acids represent the vast majority of amino acids found in proteins during translation in the ribosome, and are therefore the most essential element in biology and metabolic functions.
Images
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Functional studies - L-Amino Acid Assay Kit (ab65347)
Standard curve (fluorimetrical): mean of duplicates (+/-) with background subtracted
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Functional studies - L-Amino Acid Assay Kit (ab65347)Standard curve (colorimetrical): mean of duplicates (+/-SD) with background readings subtracted
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Functional Studies - L-Amino Acid Assay Kit (ab65347)L-Amino Acid Standard Curve.