Kinase Assay Buffer I (ab189135)
Overview
-
Product name
Kinase Assay Buffer I -
Product overview
Stock solution of buffer used for assaying kinase protein.
-
Notes
Formulation: Frozen solution of buffer containing: 25 mM MOPS, pH7.2, 12.5 mM ß-glycerol-phosphate, 25 mM MgCl2, 5 mM EGTA and 2 mM EDTA. Add to final concentration of 0.25 mM DTT prior to use. DTT solution supplied in separate vial.
Sterility: Buffer passed through a 0.2µ filter membrane and packaged aseptically.
Storage and Stability: Store product at -20°C (stable for 1 yr at -20°C from date of shipment). For optimal storage, aliquot buffer into smaller quantities after centrifugation and store at recommended temperature. For most favourable performance, avoid repeated handling and multiple freeze/thaw cycles.
Properties
-
Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 ml 5 ml 20 ml DTT (1M) 1 x 10µl 1 x 10µl 1 x 10µl Kinase Assay Buffer I 1 x 1ml 1 x 5ml 1 x 20ml -
Research areas