ID3 overexpression 293T lysate (whole cell) (ab94230)
Overview
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Product name
ID3 overexpression 293T lysate (whole cell)
See all ID3 lysates -
General notes
ab94230 is a 293T cell transfected lysate in which Human ID3 has been transiently over-expressed using a pCMV-ID3 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
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Tested applications
Suitable for: WBmore details
Properties
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Mycoplasma free
Yes -
Form
Liquid -
Storage instructions
Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles. -
Storage buffer
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Research areas
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Background
Function: ID (inhibitor of DNA binding) HLH proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding. ID-3 inhibits the binding of E2A-containing protein complexes to muscle creatine kinase E-box enhancer. May inhibit other transcription factors. Tissue specificity: Expressed abundantly in lung, kidney and adrenal gland, but not in adult brain. Similarity: Contains 1 basic helix-loop-helix (bHLH) domain.
Images
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SDS-PAGE - ID3 overexpression 293T lysate (whole cell) (ab94230)ab94230 at 15µg/lane on an SDS-PAGE gel.
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Western blot - ID3 overexpression 293T lysate (whole cell) (ab94230)All lanes : Anti-ID3 antibody (ab55269) at 1/500 dilution
Lane 1 :ID3 overexpression 293T lysate (whole cell) (ab94230)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution