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Human YWHAG (14-3-3 gamma) knockout HEK293T cell pellet (ab279125)

Overview

  • Product name

    Human YWHAG (14-3-3 gamma) knockout HEK293T cell pellet
    See all 14-3-3 gamma/YWHAG kits

  • Product overview

    Abcam’s knockout cell pellets give you access to native proteins, without the need to culture cells. Our knockout cell pellets are prepared from our single-gene knockout cell lines and provide an additional offering to our cell lysates.

    Cells are snap-frozen to provide high quality pellets that are suitable for extraction with alternative lysis buffers or for preparation of lysates from subcellular fractions. Our knockout cell pellets are suitable for a variety of applications, including PCR, gene expression profiling and DNA library preparation.

  • Parental Cell Line

    HEK293T

  • Organism

    Human

  • Mutation description

    Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon1.

  • Passage number

  • Knockout validation

    Sanger Sequencing, Western Blot (WB)

  • Notes

    Pellet size: 5 million cells/vial.

    This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

  • Tested applications

    Suitable for: WBmore details

Properties

Target

  • Function

    Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.

  • Tissue specificity

    Highly expressed in brain, skeletal muscle, and heart.

  • Sequence similarities

    Belongs to the 14-3-3 family.

  • Post-translational
    modifications

    Phosphorylated by various PKC isozymes.

  • Cellular localization

    Cytoplasm.
  • Information by UniProt

  • Alternative names

    • 14 3 3 gamma
    • 14 3 3 protein gamma
    • 14 3 3 protein gamma subtype
    • 14 3 3gamma
    • 14-3-3 protein gamma
    • 1433G_HUMAN
    • 3 monooxygenase/tryptophan 5 monooxgenase activation protein gamma polypeptide
    • KCIP 1
    • KCIP-1
    • KCIP1
    • N-terminally processed
    • Protein kinase C inhibitor protein 1
    • Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein gamma polypeptide
    • Ywhag
    see all

Properties

Images

  • Western blot - Human YWHAG (14-3-3 gamma) knockout HEK293T cell pellet (ab279125)
    Western blot - Human YWHAG (14-3-3 gamma) knockout HEK293T cell pellet (ab279125)

    Lane 1:Wild-type HEK293T cell lysate (20 ug)
    Lane 2:YWHAG knockout HEK293T cell lysate (20 ug)
    Lane 3:A431 cell lysate (20 ug)
    Lane 4:HeLa cell lysate (20 ug)

    ab137048 was shown to specifically react with 14-3-3 gamma/YWHAG in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266734 (knockout cell lysate ab257805) was used. Wild-type and 14-3-3 gamma/YWHAG knockout samples were subjected to SDS-PAGE. ab137048 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4oC at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Sanger Sequencing - Human YWHAG (14-3-3 gamma) knockout HEK293T cell pellet (ab279125)
    Sanger Sequencing - Human YWHAG (14-3-3 gamma) knockout HEK293T cell pellet (ab279125)
    Homozygous: 1 bp insertion in exon1

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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