Human XCL1 ELISA Kit (ab264620)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 28.3 pg/ml
- Range: 97.48 pg/ml - 2800 pg/ml
- Sample type: Cell culture media, Cell culture supernatant, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human XCL1 ELISA Kit
See all XCL1 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Supernatant 8 5.4% Inter-assay Sample n Mean SD CV% Supernatant 3 3.1% -
Sample type
Cell culture supernatant, Serum, Cell culture media, Hep Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
28.3 pg/ml -
Range
97.48 pg/ml - 2800 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 105 101% - 109% Serum 98 96% - 100% Cell culture media 90 89% - 92% Hep Plasma 87 85% - 88% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human XCL1 ELISA Kit (ab264620) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of XCL1 protein in cell culture supernatant, hep plasma, serum, and cell culture media. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human XCL1 with 28.3 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
XCL1 (Lymphotactin) is a secreted glycosylated protein of C chemokine family. It plays a role in chemotactic activity of lymphocytes, but not monocytes or neutrophils. In the thymus, XCL1 mediates medullary accumulation of dendritic cells and contributes to T cell development which plays a role in establishment of self-tolerance. XCL2 is functionally related XCL1 homolog, these proteins share 98% amino acids sequence identity.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human XCL1 Capture Antibody 1 x 600µl 10X Human XCL1 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml Human XCL1 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent 50BS 1 x 20ml Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Relevance
Human lymphotactin (Lptn)/XCL1, also named human SCM1 and ATAC, is a member of gamma or C subfamily of chemokines that lack two of the four invariant cysteine residues normally found in the CC and CXC chemokines and have an extended C-terminus. Human lymphotactin encodes a 114 amino acid precursor protein with a 21 amino acid predicted signal peptide. The gene for human and mouse lymphotactin has been mapped to chromosome 1. Human and mouse lymphotactin share approximately 60% amino acid sequence identity. The expression of lymphotactin is restricted to activated T cells such as activated CD8+ T cells and other class I MHC restricted T cells. Since lymphotactin is produced by lymphocytes and acts on lymphocytes, it is speculated that it is a messenger in T cell chemoattraction. Human lymphotactin is chemotactic for lymphocytes and NK cells. Its expression is abundant in some activated T cells such as activated CD8+ T cells and other class I MCH restricted T cells and is absent in CD4+ T cells. It has been shown that lymphotactin is a key regulator of lymphocyte motility and adhesion during acute allograft rejection. The specific receptor for lymphotactin is the orphan receptor XCR1 (GPR5). It has chemotactic activity for lymphocytes and natural killer cells but not for monocytes or neutrophils. -
Cellular localization
Secreted -
Alternative names
- ATAC
- Chemokine (C motif) ligand 1
- Cytokine SCM1
see all -
Database links
- Entrez Gene: 6375 Human
- Omim: 600250 Human
- SwissProt: P47992 Human
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Human XCL1 standard curve comparisonStandard Curve comparison between human XCL1 SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
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Example of human XCL1 standard curve in Sample Diluent NS.The XCL1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native XCL1 in human PMA + Ionomycin treated PBMC cell culture supernatant samplesPeripheral blood mononuclear cells (PBMC) were cultured in the presence (stimulated) or absence of 50 ng/mL PMA and 1 µg/mL Ionomycin for 24 hours. Cell culture supernatants were collected and assayed for the presence of XCL1. The concentrations of XCL1 were measured in duplicates, interpolated from the XCL1 standard curves and corrected for sample dilution. Undiluted samples are as follows: PBMC cell culture supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean XCL1 concentration was determined to be 4,506 pg/mL in PMA + Ionomycin treated PBMC cell culture supernatant and not detectable in untreated samples (data not shown).
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Sandwich ELISA - Human XCL1 ELISA Kit (ab264620)To learn more about the advantages of recombinant antibodies see here.
