Human Visfatin ELISA Kit (ab267658)
Key features and details
- Sensitivity: 1.1 ng/ml
- Range: 1.1 ng/ml - 300 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
Overview
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Product name
Human Visfatin ELISA Kit
See all Visfatin kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
1.1 ng/ml -
Range
1.1 ng/ml - 300 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 72 69% - 80% Plasma 79 69% - 94% Cell culture media 108 91% - 119% -
Assay duration
Multiple steps standard assay -
Product overview
Human Visfatin ELISA Kit is designed for the quantitative determination of Visfatin in cell culture supernatants, plasma and serum samples.
ΔNote: Human Visfatin concentration is low in normal serum/plasma, and may not be detectable in this assay.
This assay employs an antibody specific for human Visfatin coated on a 96-well plate. Standards and samples are pipetted into the wells and Visfatin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human Visfatin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Visfatin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
This ELISA kit shows no cross-reactivity with the following cytokines tested: Human Coagulation Factor X, CHMP2B, Contactin-3, Cortactin, CrkL, Cyr61/CCN1, DAPP1, Dynactin Subunit 1/DCTN1, DFF45/ICAD, DRAK1, GRAP2, GRK5, Hydroxyacid Oxidase1/HAO-1, LRIG1, MMP-12, NCK1, Nectin-2/CD112, Nesfatin-1/Nucleobindin-2, Neurogranin, Nrf2, NUDT5, PAR1, Pancreatic Polypeptide/PP, Peroxiredoxin 2, PSMA1, PU.1/Spi-1, RalA, RCOR1/CoREST, Serpin B8, SH2D1A, SHP-1, Siglec-6/CD327, SorCS3, THAP11, ULBP4/RAET1E, UNC5H3, VAMP-1, VAMP-2.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 250X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml 5X Assay Diluent D 1 x 15ml Anti-Human Visfatin coated Microplate (12 x 8 wells) 1 unit Assay Diluent A 1 x 30ml Biotinylated Anti-Human Visfatin Detection Antibody 2 vials Human Visfatin Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
Research areas
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Function
Catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD. It is the rate limiting component in the mammalian NAD biosynthesis pathway. -
Tissue specificity
Expressed in large amounts in bone marrow, liver tissue, and muscle. Also present in heart, placenta, lung, and kidney tissues. -
Pathway
Cofactor biosynthesis; NAD(+) biosynthesis; nicotinamide D-ribonucleotide from 5-phospho-alpha-D-ribose 1-diphosphate and nicotinamide: step 1/1. -
Sequence similarities
Belongs to the NAPRTase family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Alternative names
- 1110035O14Rik
- AI314458
- AI480535
see all
Images
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Example Standard Curve