Lane 1: Wild-type A549 cell lysate (20µg)

Lane 2: MAP3K11 knockout A549 cell lysate (20µg)
Lanes 1- 2: Merged signal (red and green). Green - ab51068 observed at 105 kDa. Red - loading control ab8245 observed at 37 kDa.
ab51068 Anti-MLK3 antibody [EP1460Y] was shown to specifically react with MLK3 in wild-type A549 cells in western blot. Loss of signal was observed when knockout cell line ab267169 (knockout cell lysate ab257519) was used. Wild-type and MLK3 knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab51068 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4^°C at 1 in 5000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

Allele-1: 288 bp insertion in exon5

 

Allele-2: 1 bp deletion in exon5

 

Allele-3: 1 bp insertion in exon5