Human VAP-1 ELISA Kit (ab243684)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 8.7 pg/ml
- Range: 31.25 pg/ml - 2000 pg/ml
- Sample type: Cell culture media, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
-
Product name
Human VAP-1 ELISA Kit
See all VAP1 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% serum 8 4.8% Inter-assay Sample n Mean SD CV% serum 3 2.9% -
Sample type
Serum, Cell culture media, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
8.7 pg/ml -
Range
31.25 pg/ml - 2000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 105 99% - 109% Cell culture media 96 93% - 97% Hep Plasma 100 91% - 106% EDTA Plasma 97 96% - 98% Cit plasma 105 97% - 115% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human VAP-1 ELISA Kit (ab243684) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of VAP-1 protein in cit plasma, edta plasma, hep plasma, serum, and cell culture media. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human VAP-1 with 8.7 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
-
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
-
Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human VAP-1 Capture Antibody 1 x 600µl 10X Human VAP-1 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 5BI 1 x 6ml Human VAP-1 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
-
Function
Cell adhesion protein that participates in lymphocyte recirculation by mediating the binding of lymphocytes to peripheral lymph node vascular endothelial cells in an L-selectin-independent fashion. Has a monoamine oxidase activity. May play a role in adipogenesis. -
Tissue specificity
Strongly expressed on the high endothelial venules of peripheral lymph nodes and on hepatic endothelia. Also highly expressed in appendix, lung and small intestine. Expressed also in adipose tissue, in bone marrow, colon, heart, kidney, ovary, pancreas, placenta, prostate, skeletal muscle, spleen and testis. -
Sequence similarities
Belongs to the copper/topaquinone oxidase family. -
Post-translational
modificationsTopaquinone (TPQ) is generated by copper-dependent autoxidation of a specific tyrosyl residue.
N- and O-glycosylated. -
Cellular localization
Membrane. - Information by UniProt
-
Alternative names
- Amine oxidase, copper containing 3 (vascular adhesion protein 1)
- AOC3
- AOC3_HUMAN
see all -
Database links
- Entrez Gene: 8639 Human
- Omim: 603735 Human
- SwissProt: Q16853 Human
- Unigene: 198241 Human
Images
-
Other - Human VAP-1 ELISA Kit (ab243684)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
-
Human VAP-1 Standard Curve Comparison.Standard Curve comparison between human VAP-1 SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
-
Example of human VAP-1 standard curve in Sample Diluent NS.The VAP-1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
-
Interpolated concentrations of native VAP-1 in human serum and plasma samples.The concentrations of VAP-1 were measured in duplicates, interpolated from the VAP-1 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:400, plasma (citrate) 1:200, plasma (EDTA) 1:200 and plasma (heparin) 1:400. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean VAP-1 concentration was determined to be 238.1 ng/mL in serum, 183.6 ng/mL in plasma (citrate), 196.6 ng/mL in plasma (EDTA) and 279.4 ng/mL in plasma (heparin).
-
Interpolated concentrations of spike VAP-1 in cell culture media samples.The concentrations of VAP-1 were measured in duplicates, interpolated from the VAP-1 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
-
Serum from ten individual healthy human male donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean VAP-1 concentration where detectable was determined to be 512.9 ng/mL with a range of 0 – 855.8 ng/mL when diluted to 0.1% serum.
-
Serum from ten individual healthy human female donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean VAP-1 concentration was determined to be 345.5 ng/mL with a range of 183.1 – 454.8 ng/mL.
-
Sandwich ELISA - Human VAP-1 ELISA Kit (ab243684)To learn more about the advantages of recombinant antibodies see here.
