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Human ULBP-1 ELISA Kit (ab267647)

Key features and details

  • Sensitivity: 0.4 ng/ml
  • Range: 0.41 ng/ml - 100 ng/ml
  • Sample type: Cell culture supernatant, Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

  • Product name

    Human ULBP-1 ELISA Kit

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall
    Inter-assay
    Sample n Mean SD CV%
    Overall

  • Sample type

    Cell culture supernatant, Serum, Plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    0.4 ng/ml

  • Range

    0.41 ng/ml - 100 ng/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 75.25 68% - 82%
    Plasma 72.65 67% - 89%
    Cell culture media 76.22 71% - 83%

  • Assay duration

    Multiple steps standard assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    Human ULBP-1 ELISA Kit is designed for the quantitative determination of ULBP-1 in cell culture supernatants, plasma and serum samples.


    Δ Note: Human ULBP-1 concentration is low in normal serum and plasma, and may not be detectable in this assay.


    This assay employs an antibody specific for human ULBP-1 coated on a 96-well plate. Standards and samples are pipetted into the wells and ULBP-1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human ULBP-1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of ULBP-1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


    This ELISA kit shows no cross-reactivity with the following cytokines tested: Human ADAM-12, ADAM-8, B7-H3, BMPR-IB, Cadherin-13, Cadherin-4, CD155, CD229, CD48, CD58, CD84, CD99, CEACAM-5, Coagulation Factor XIV, Cystatin A, Cystatin B, Cystatin E/M, Desmoglein-2, DR3, ErbB4, ESAM, FGF-21, Galectin-2, Galectin-9, ICOS, JAM-A, JAM-B, Kallikrein 5, Midkine, Pentraxin-3, Pref-1, Siglec-10, SLAM, SP-D, Syndecan-4, Testican 2, TIM-3, TLR4, TRAIL.

  • Platform

    Microplate

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer 1 x 25ml
    400X HRP-Streptavidin Concentrate 1 x 200µl
    5X Assay Diluent B 1 x 15ml
    Anti-Human ULBP-1 coated Microplate (12 x 8 wells) 1 unit
    Assay Diluent C 1 x 30ml
    Biotinylated Anti-Human ULBP-1 Detection Antibody 2 vials
    Human ULBP-1 Standard (Lyophilized) 2 vials
    Stop Solution 1 x 8ml
    TMB Substrate Solution 1 x 12ml

  • Research areas

  • Function

    Ligand for the NKG2D receptor, together with at least ULBP2 and ULBP3. ULBPs activate multiple signaling pathways in primary NK cells, resulting in the production of cytokines and chemokines. Binding of ULBPs ligands to NKG2D induces calcium mobilization and activation of the JAK2, STAT5, ERK and PI3K kinase/Akt signal transduction pathway. In CMV infected cells, interacts with soluble CMV glycoprotein UL16. The interaction with UL16 blocked the interaction with the NKG2D receptor, providing a mechanism by which CMV infected cells might escape the immune system. UL16 also causes ULBP1 to be retained in the ER and cis-Golgi apparatus so that it does not reach the cell surface.

  • Tissue specificity

    Expressed in T-cells, B-cells, erythroleukemia cell lines and in a wide range of tissues including heart, brain, lung, liver, testis, lymph node, thymus, tonsil and bone marrow. Also found in fetal heart, brain, lung and liver.

  • Sequence similarities

    Belongs to the MHC class I family.

  • Cellular localization

    Cell membrane. Endoplasmic reticulum. Detected intracellularly in the endoplasmic reticulum of CMV-infected fibroblasts.
  • Information by UniProt

  • Alternative names

    • Alcan beta
    • ALCAN-beta
    • N2DL-1
    • N2DL1
    • N2DL1_HUMAN
    • NKG2D ligand 1
    • NKG2D ligand 1 precursor
    • NKG2DL1
    • RAET1I
    • Retinoic acid early transcript 1I
    • UL16
    • UL16 binding protein 1
    • UL16-binding protein 1
    • ULBP1
    see all

  • Database links

    Images

    • Example data
      Example data

      This standard curve is for demonstration only. A standard curve must be run with each assay.

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