Human TSC-1 ELISA Kit (ab223594)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 1.54 pg/ml
- Range: 4.69 pg/ml - 300 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
-
Product name
Human TSC-1 ELISA Kit
See all TSC-1 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Supernatant 8 4.8% Inter-assay Sample n Mean SD CV% Supernatant 3 8.7% -
Sample type
Cell culture supernatant, Serum, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
1.54 pg/ml -
Range
4.69 pg/ml - 300 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 101 95% - 108% Serum 102 90% - 118% EDTA Plasma 101 85% - 109% Cit plasma 102 83% - 117% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human TSC-1 ELISA Kit (ab223594) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of TSC-1 protein in cell culture supernatant, cit plasma, edta plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human TSC-1 with 1.54 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
-
Notes
TSC-1 (Eotaxin 3 / CCL26) is a small cytokine within the CC chemokine family coded by a gene located on human chromosome 7. TSC-1 is also know by Macrophage inflammatory protein 4-alpha, Thymic stroma chemokine-1 and IMAC. It has known expression in heart, lung and ovarian tissue. TSC-1 expression can be induced by IL-4 stimulation which then can activate chemotaxic activity of eosinophils and basophiles by binding to the cell surface chemokine receptor CCR3.
-
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
-
Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BI 1 x 6ml 10X Human TSC-1 Detector Antibody 1 x 600µl 10X Human TSC-1 Capture Antibody 1 x 600µl Human TSC-1 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
-
Function
Chemotactic for eosinophils and basophils. Binds to CCR3. -
Tissue specificity
Ubiquitously expressed at low levels in various tissues including heart and ovary. -
Sequence similarities
Belongs to the intercrine beta (chemokine CC) family. -
Cellular localization
Secreted. - Information by UniProt
-
Alternative names
- C-C motif chemokine 26
- CC chemokine IMAC
- CCL 26
see all -
Database links
- Entrez Gene: 10344 Human
- Omim: 604697 Human
- SwissProt: Q9Y258 Human
- Unigene: 131342 Human
Images
-
Other - Human TSC-1 ELISA Kit (ab223594)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
-
Example of Human Eotaxin 3 standard curve in Sample Diluent NS.Background-subtracted data values (mean +/- SD) are graphed.
-
Interpolated concentrations of native Eotaxin 3 in human A549 cell culture supernatant samples.The concentrations of Eotaxin 3 were measured in duplicates, interpolated from the Eotaxin 3 standard curves and corrected for sample dilution. Undiluted human A549 supernatant: 20%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Eotaxin 3 concentration was determined to be 5559.17 pg/mL.
-
Interpolated concentrations of spiked Eotaxin 3 in serum and plasma.Interpolated concentrations of spiked Eotaxin 3 in human serum 50%, plasma (citrate) 50% and plasma (EDTA) 50%. The concentrations of Eotaxin 3 were measured in duplicate and interpolated from the Eotaxin 3 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Eotaxin 3 concentration was determined to be 161 pg/mL in human serum and 320.02 pg/mL in citrate plasma, 318.05 pg/ml in EDTA plasma.
-
Sandwich ELISA - Human TSC-1 ELISA Kit (ab223594)To learn more about the advantages of recombinant antibodies see here.