Human Trypsin 2 ELISA Kit (ab267611)
Key features and details
- Sensitivity: 25 pg/ml
- Range: 24.58 pg/ml - 6000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Trypsin 2 ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
25 pg/ml -
Range
24.58 pg/ml - 6000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 119.2 97% - 142% Plasma 114.7 86% - 143% Cell culture media 99.72 96% - 104% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human Trypsin 2 ELISA Kit is designed for the quantitative determination of Trypsin 2 in cell culture supernatants, plasma and serum samples.
This assay employs an antibody specific for human Trypsin 2 coated on a 96-well plate. Standards and samples are pipetted into the wells and Trypsin 2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human Trypsin 2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Trypsin 2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml 5X Assay Diluent D 1 x 15ml Anti-Human Trypsin 2 coated Microplate (12 x 8 wells) 1 unit Biotinylated Anti-Human Trypsin 2 Detection Antibody 2 vials Human Trypsin 2 Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
Research areas
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Sequence similarities
Belongs to the peptidase S1 family.
Contains 1 peptidase S1 domain. -
Post-translational
modificationsSulfated on tyrosine. -
Cellular localization
Secreted > extracellular space. - Information by UniProt
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Alternative names
- Anionic trypsinogen
- EC 3.4.21.4
- MGC111183
see all -
Database links
- Entrez Gene: 5645 Human
- Omim: 601564 Human
- SwissProt: P07478 Human
- Unigene: 449281 Human
- Unigene: 728780 Human
Images
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Example data
This standard curve is for demonstration only. A standard curve must be run with each assay.
