Human TNF alpha ELISA Kit (ab46087)
Key features and details
- Sensitivity: 10 pg/ml
- Range: 25 pg/ml - 800 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human TNF alpha ELISA Kit
See all TNF alpha kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Sample 6 3.3% Inter-assay Sample n Mean SD CV% Sample 6 9% -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
= 10 pg/ml -
Range
25 pg/ml - 800 pg/ml -
Recovery
107 %
Sample specific recovery Sample type Average % Range Serum 81 74% - 90% -
Assay time
3h 40m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human TNF alpha ELISA kit (ab46087) is a quantitative ELISA assay for the measurement of TNF alpha in supernatants, buffered solutions, serum and plasma samples.
A monoclonal antibody specific TNF alpha has been coated onto the wells of the microtiter strips provided. Samples, including standards of known TNF alpha concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for TNF alpha are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of TNF alpha present in the samples.
This kit will recognize both endogenous and recombinant Human TNF alpha.
Get results in 90 minutes with Human TNF alpha ELISA Kit (ab181421) from our SimpleStep ELISA® range.
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components Identifier 1 x 96 tests 2 x 96 tests 10X Standard Diluent Buffer Black 1 x 15ml 1 x 25ml 200X Wash Buffer White 1 x 10ml 2 x 10ml Biotinylated Antibody Diluent Red 1 x 7.5ml 1 x 13ml Biotinylated anti-TNF alpha Red 1 x 400µl 2 x 400µl Chromogen TMB Substrate Solution 1 x 11ml 1 x 24ml HRP Diluent Red 1 x 12ml 1 x 23ml Plastic Plate Covers 1 x 2 units 1 x 4 units Standard Diluent (Serum) 1 x 7ml 2 x 7ml Stop Reagent Black 1 x 11ml 2 x 11ml Streptavidin-HRP 2 x 5µl 4 x 5µl TNF alpha Control Yellow 2 vials 4 vials TNF alpha Microplate (12 x 8 well strips) 1 unit 2 units TNF alpha Standard 2 vials 4 vials -
Research areas
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Function
Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. -
Involvement in disease
Genetic variations in TNF are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis). -
Sequence similarities
Belongs to the tumor necrosis factor family. -
Post-translational
modificationsThe soluble form derives from the membrane form by proteolytic processing.
The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Alternative names
- APC1
- APC1 protein
- Cachectin
see all -
Database links
- Entrez Gene: 7124 Human
- Omim: 191160 Human
- SwissProt: P01375 Human
- Unigene: 241570 Human
Images
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Typical Standard Curve
Representative standard curve using ab46087
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Sandwich ELISA - TNF alpha Human ELISA Kit (ab46087)TNF? was measured in cell culture medium from U937 control cells, or cells stimulated with LPS (1 µg x mL-1; 6 h) or PMA (10 ng x mL-1; 24 h) with LPS and control and stimulated PBMCs (PHA 10 µg x mL-1; 3 days). Results are shown with background signal subtracted (duplicates +/- SD), with the positive control from ab46087 included.
