Human Thrombin Antithrombin Complex total antigen assay ELISA kit (ab279724)
Key features and details
- Sensitivity: 0.718 ng/ml
- Range: 1 ng/ml - 1000 ng/ml
- Sample type: Cit plasma, EDTA Plasma, Hep Plasma, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Quantitative
- Reacts with: Human
Overview
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Product name
Human Thrombin Antithrombin Complex total antigen assay ELISA kit -
Detection method
Colorimetric -
Sample type
Serum, Plasma, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Quantitative -
Sensitivity
0.718 ng/ml -
Range
1 ng/ml - 1000 ng/ml -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human Thrombin Antithrombin (TAT) Complex total antigen assay ELISA kit is intended for the quantitative determination of the covalent complex of thrombin and its inhibitor antithrombin III in human plasma and serum.
Human TAT Complex will bind to the affinity purified Anti-Human Thrombin capture antibody coated on the microtiter plate. After appropriate washing steps, monoclonal Anti-Human Antithrombin primary antibody binds to the TAT Complex captured on the plate. Excess antibody is washed away and bound monoclonal antibody is reacted with the secondary antibody conjugated to HRP. Following an additional washing step, TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of Human TAT complex. Color development is proportional to the concentration of TAT complex in the samples.
Free Thrombin, Prothrombin and Antithrombin III will not be detected by this assay.
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Tested applications
Suitable for: ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 96 tests 10X Wash Buffer 1 x 50ml Anti-Human Antithrombin Primary Antibody Lyophilized Vial 1 vial Anti-Human Thrombin ELISA Plate 1 unit Anti-Mouse HRP-conjugated Secondary Antibody 1 vial Human TAT complex Standard Lyophilized Vial 1 vial TMB Substrate 1 x 10ml -
Research areas
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Relevance
Thrombin-antithrombin (TAT) complexes formed following the neutralization of thrombin by AT have been used as a surrogate marker for thrombin generation; serial changes in TAT levels have been used to determine alterations of the extent of hemostatic activation in the course of a disease or to assess the impact of specific therapy (for example, the effect of heparin in ameliorating disseminated intravascular coagulation [DIC]). -
Cellular localization
Secreted -
Alternative names
- TAT
Images
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Linear range exaple data
A typical standard curve for Human TAT complex (linear range). Example only.
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Full range example dataA typical standard curve for Human TAT complex (full range). Example only.