Human TFPI ELISA Kit (ab274392)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 21.4 pg/ml
- Range: 125 pg/ml - 8000 pg/ml
- Sample type: Cell culture media, Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human TFPI ELISA Kit
See all TFPI kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 6.5% Inter-assay Sample n Mean SD CV% Serum 3 6.9% -
Sample type
Cell culture supernatant, Urine, Serum, Cell culture media, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
21.4 pg/ml -
Range
125 pg/ml - 8000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 106 110% - 112% Urine 110 105% - 117% Serum 115 111% - 119% Cell culture media 91 88% - 94% Hep Plasma 117 113% - 120% EDTA Plasma 116 114% - 117% Cit plasma 102 96% - 106% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human TFPI ELISA kit (ab274392) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human TFPI protein in serum, plasma, urine and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human TFPI with 21.4 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Tissue factor pathway inhibitor (TFPI), also known as extrinsic pathway inhibitor (EPI) or Lipoprotein-associated coagulation inhibitor (LACI), inhibits coagulation through the formation of a complex with coagulation factor X, VII, and tissue factor (TF). TFPI functions to prevent the activation of factor X which thus preventing clot formation. Active TFPI is a 40kDa full-length protein is found 50% in LDL, 40% in LHDL, and 10% in VLDL. Abnormally high levels of TFPI are thought to lead to severe hemostatic disturbances such as thrombosis, disseminated intravascular coagulation, and septic shock. Similarly, TFPI is also used clinically to treat inflammatory and cardiovascular diseases where clot formation is a high prevalence.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human TFPI Capture Antibody 1 x 600µl 10X Human TFPI Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 5BI 1 x 6ml Human TFPI Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Inhibits factor X (X(a)) directly and, in a Xa-dependent way, inhibits VIIa/tissue factor activity, presumably by forming a quaternary Xa/LACI/VIIa/TF complex. It possesses an antithrombotic action and also the ability to associate with lipoproteins in plasma. -
Tissue specificity
Mostly in endothelial cells. -
Sequence similarities
Contains 3 BPTI/Kunitz inhibitor domains. -
Domain
This inhibitor contains three inhibitory domains. The first domain interacts with VIIa and TF, the second one with Xa. -
Post-translational
modificationsO-glycosylated. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Anti convertin
- EPI
- Extrinsic pathway inhibitor
see all -
Database links
- Entrez Gene: 7035 Human
- Omim: 152310 Human
- SwissProt: P10646 Human
- Unigene: 516578 Human
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Human TFPI competitor curve comparisonStandard Curve comparison between Human TFPI SimpleStep ELISA kit and traditional ELISA kit from leading competitor.
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Human TFPI standard curve comparisonStandard Curve comparison between the original Human TFPI SimpleStep ELISA kit (ab188392) and current Human TFPI SimpleStep ELISA kit (ab274392).
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Example of human TFPI standard curve in Sample Diluent NS.The TFPI standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native TFPI in human serum and plasma.The concentrations of TFPI were measured in duplicates, interpolated from the TFPI standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1%, plasma (citrate) 1%, plasma (EDTA) 1% and plasma (Heparin) 1%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TFPI concentration was determined to be 415.32 ng/mL in serum, 336.93 ng/mL in plasma (citrate), 421.32 ng/mL in plasma (EDTA), and 335.79 ng/mL in plasma (Heparin).
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Interpolated concentrations of native TFPI in human urine and cell culture supernatant samples.The concentrations of TFPI were measured in duplicates, interpolated from the TFPI standard curves and corrected for sample dilution. Undiluted samples are as follows: Urine 50% and HepG2 cell culture supernatant 0.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TFPI concentration was determined to be 8.55 ng/mL in urine, and 971.14 ng/mL in HepG2 supernatant.
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0.5% Serum from ten individual healthy human female donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TFPI concentration was determined to be 502.80 ng/mL with a range of 290.26 – 698.50 ng/mL.
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Sandwich ELISA - Human TFPI ELISA Kit (ab274392)To learn more about the advantages of recombinant antibodies see here.
