Human TFF2 ELISA Kit (ab275553)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 3.4 pg/ml
- Range: 9.375 pg/ml - 600 pg/ml
- Sample type: Cit plasma, EDTA Plasma, Hep Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human TFF2 ELISA Kit
See all TFF2 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Human Serum 8 4.3% Inter-assay Sample n Mean SD CV% Human Serum 3 5.3% -
Sample type
Urine, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
3.4 pg/ml -
Range
9.375 pg/ml - 600 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Urine 100 96% - 103% Serum 100 95% - 103% Tissue Extracts 99 95% - 103% Hep Plasma 95 93% - 97% EDTA Plasma 82 80% - 87% Cit plasma 94 91% - 101% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human TFF2 ELISA kit (ab275553) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human TFF2 protein in serum, plasma, urine, and cell and tissue extract. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human TFF2 with 3.40 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
TFF2 is a member of the trefoil factor (TFF) family which contains three secreted, protease-resistant polypeptides that help to maintain gastrointestinal system integrity. Family members are 7-12 kDa in size and contain a 3-disulfide bonds. TFF1, 2, and 3 are expressed in specific areas of the digestive tract, and promote restitution, or the resealing of superficial wounds after injury. TFF2 Inhibits gastrointestinal motility and gastric acid secretion. It has been reported to function as a structural component of gastric mucus, possibly by stabilizing glycoproteins in the mucus gel through interactions with carbohydrate side chains.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human TFF2 Capture Antibody 1 x 600µl 10X Human TFF2 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 5BI 1 x 6ml Human TFF2 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Inhibits gastrointestinal motility and gastric acid secretion. Could function as a structural component of gastric mucus, possibly by stabilizing glycoproteins in the mucus gel through interactions with carbohydrate side chains. -
Tissue specificity
Stomach. -
Sequence similarities
Contains 2 P-type (trefoil) domains. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- SML 1
- SML1
- SP
see all -
Database links
- Entrez Gene: 7032 Human
- Omim: 182590 Human
- SwissProt: Q03403 Human
- Unigene: 2979 Human
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human TFF2 standard curve in Sample Diluent NS.The TFF2 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Example of human TFF2 standard curve in 1X Cell Extraction Buffer PTR.The TFF2 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native TFF2 in human serum, plasma and urine samples.The concentrations of TFF2 were measured in duplicates, interpolated from the TFF2 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 10%, plasma (citrate) 20%, plasma (EDTA) 20%, plasma (heparin) 20%, and pooled urine 1:800. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TFF2 concentration was determined to be 4.53 ng/mL in serum, 2.45 ng/mL in plasma (citrate), 2.64 ng/mL in plasma (EDTA), 1.95 ng/mL in plasma (heparin), and 394.66 ng/mL in urine.
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Interpolated concentrations of native TFF 2 in human stomach extract 0.5 µg/mL extract load.The concentrations of TFF2 were measured in duplicate and interpolated from the TFF2 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TFF2 concentration was determined to be 338.99 pg/mL in stomach extract.
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5% Serum from ten individual healthy human female donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TFF2 concentration was determined to be 3.78 ng/mL with a range of 0.71 – 8.45 ng/mL.
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Sandwich ELISA - Human TFF2 ELISA Kit (ab275553)To learn more about the advantages of recombinant antibodies see here.
