Human Syndecan 3 ELISA Kit (ab267607)
Key features and details
- Sensitivity: 60 pg/ml
- Range: 0.061 ng/ml - 15 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Syndecan 3 ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% overall Inter-assay Sample n Mean SD CV% overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
60 pg/ml -
Range
0.061 ng/ml - 15 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 96.55 84% - 112% Plasma 86.82 77% - 99% Cell culture media 81.84 73% - 97% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human Syndecan 3 ELISA Kit is designed for the quantitative determination of Syndecan 3 in cell culture supernatants, plasma and serum samples.
Δ Note: Human Syndecan 3 concentration is low in normal serum/plasma and may not be detectable in this assay.
This assay employs an antibody specific for human Syndecan 3 coated on a 96-well plate. Standards and samples are pipetted into the wells and Syndecan 3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human Syndecan 3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Syndecan 3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
This ELISA kit shows no cross-reactivity with the following cytokines tested: human ADAMTS-13, Aggrecan, Angiotensinogen (SERPINA8), B7-H1 (CD274), BMPR-IA (ALK-3), BMPR-II, Cadherin-11, CD27 (TNFRSF7), CD6, Ck beta 8-1 (CCL23), CNTF, DNAM-1 (CD226), CD147 (EMMPRIN), FLRG, Follistatin-like 1, Fractalkine, Galectin-1, GITR Ligand (TNFSF18), Granulysin (NKG5/LAG-2), IL-1 R3 (IL-1 R Acp), IL-15 R alpha, IL-17E (IL-25), IL-32 alpha, CHL-1 (L1CAM-2), LRIG3, LRP-6, MEPE (OF45), Nectin-4, Periostin, Persephin, Renin, RGM-B, ROBO3, S100 A8, Siglec-7 (CD328), Thrombospondin 2, Thrombospondin 5, Tie-1, ULBP-2.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 100X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent 1 x 15ml Anti-Human Syndecan 3 coated Microplate (12 x 8 wells) 1 unit Biotinylated Anti-Human Syndecan 3 Detection Antibody 2 vials Human Syndecan 3 Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
Research areas
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Function
Cell surface proteoglycan that may bear heparan sulfate (By similarity). May have a role in the organization of cell shape by affecting the actin cytoskeleton, possibly by transferring signals from the cell surface in a sugar-dependent mechanism. -
Tissue specificity
Expressed in the nervous system, the adrenal gland, and the spleen. -
Sequence similarities
Belongs to the syndecan proteoglycan family. -
Post-translational
modificationsO-glycosylated within the Thr/Ser-rich region which could interact with lectin domains on other molecules. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- N-syndecan
- Sdc3
- SDC3_HUMAN
see all -
Database links
- Entrez Gene: 9672 Human
- Omim: 186357 Human
- SwissProt: O75056 Human
- Unigene: 158287 Human
Images
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Example data
This standard curve is for demonstration only. A standard curve must be run with each assay.