Call: +7 771 977 66 65, +7 705 421 2277
Sign in or Register
My basket

Astana Biomed Group, an authorized Abcam distributor in Central Asia

Abiomed homepage

  • Categories
    Signal Transduction
    Cancer
    Epigenetics and Nuclear Signaling
    Immunology
    Cell Biology
    Cardiovascular
    Neuroscience
    Tags & Cell Markers
    Kits/ Lysates/ Other
    Developmental Biology
    Microbiology
    Biochemicals
    Secondary antibodies
    Isotype/Loading Controls
    Antibody Arrays
  • About us
  • Partners
  • Contact
    Address

    Saryarka 32, 18, 010000, Astana city, Kazakhstan

    Telephone +7 771 977 66 65, +7 705 421 2277

    Email

    laboratory@ctlab.kz, orders@abiomed.kz

Back to category
Signal Transduction Signaling Pathway G Protein Signaling Small G Proteins Other

Human SPG7 (PGN) knockout HeLa cell lysate (ab258202)

Price and availability

318 288 ₸

Availability

Order now and get it on Friday March 05, 2021

Human SPG7 (PGN) knockout HeLa cell lysate (ab258202)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

You may also be interested in

Product image
Anti-PAR1/Thrombin Receptor antibody - BSA and Azide free (ab281125)
Product image
Anti-LYPD3 antibody [EPR9106] (ab155080)
Product image
Anti-CtIP antibody (ab117722)
Product image
Anti-PITPNC1 antibody (ab238643)

Overview

  • Product name

    Human SPG7 (PGN) knockout HeLa cell lysate
    See all SPG7/PGN kits
  • Product overview


    Knockout cell lysate achieved by CRISPR/Cas9.

  • Parental Cell Line

    HeLa
  • Organism

    Human
  • Mutation description

    Knockout achieved by using CRISPR/Cas9, 2 bp deletion in exon2 and 5 bp deletion in exon2.
  • Passage number

  • Knockout validation

    Sanger Sequencing
  • Reconstitution notes

    To use as WB control, resuspend the lyophilizate in 50 µL of LDS* Sample Buffer to have a final concentration of 2 mg/ml. For reducing conditions, we recommend a final concentration of 0.1 M DTT.

    *Usage of SDS sample buffer is not recommended with these lyophilized lysates.

  • Notes

    Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version - found here. Please refer to our lysis protocol for further details on how our lysates are prepared.

    User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

    Access thousands of knockout cell lysates, generated from commonly used cancer cell lines.
    See here for more information on knockout cell lysates.

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

    This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

Properties

  • Storage instructions

    Store at -80°C. Please refer to protocols.
  • Components 1 kit
    ab263001 - Human SPG7 knockout HeLa cell lysate 1 x 100µg
    ab255929 - Human wild-type HeLa cell lysate 1 x 100µg
  • Research areas

    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Other
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteolytic enzymes
    • Other proteases
  • Cell type

    epithelial
  • Disease

    Adenocarcinoma
  • Gender

    Female
  • STR Analysis

    Amelogenin X D5S818: 11, 12 D13S317: 12, 13.3 D7S820: 8, 12 D16S539: 9, 10 vWA: 16, 18 TH01: 7 TPOX: 8,12 CSF1PO: 9, 10

Target

  • Target information above from: UniProt accession Q9UQ90 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • CAR
    • Cell adhesion regulator
    • Cell matrix adhesion regulator
    • CMAR
    • Paraplegin
    • PGN
    • Spastic paraplegia 7 (pure and complicated autosomal recessive)
    • Spastic paraplegia 7 homolog (human)
    • Spastic paraplegia 7 protein
    • Spastic paraplegia 7, paraplegin (pure and complicated autosomal recessive)
    • Spastic paraplegia protein 7
    • SPG5C
    • Spg7
    • SPG7 gene
    • SPG7_HUMAN
    see all

Properties

  • Storage instructions

    Store at -80°C. Please refer to protocols.
  • Components 1 kit
    ab263001 - Human SPG7 knockout HeLa cell lysate 1 x 100µg
    ab255929 - Human wild-type HeLa cell lysate 1 x 100µg
  • Research areas

    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Other
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteolytic enzymes
    • Other proteases
  • Cell type

    epithelial
  • Disease

    Adenocarcinoma
  • Gender

    Female
  • STR Analysis

    Amelogenin X D5S818: 11, 12 D13S317: 12, 13.3 D7S820: 8, 12 D16S539: 9, 10 vWA: 16, 18 TH01: 7 TPOX: 8,12 CSF1PO: 9, 10

Images

  • Sanger Sequencing - Human SPG7 knockout HeLa cell lysate (ab258202)
    Sanger Sequencing - Human SPG7 knockout HeLa cell lysate (ab258202)

    Allele-1: 5 bp deletion in exon2

     

  • Sanger Sequencing - Human SPG7 knockout HeLa cell lysate (ab258202)
    Sanger Sequencing - Human SPG7 knockout HeLa cell lysate (ab258202)

    Allele-2: 2 bp deletion in exon2

     

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Clear all

Recently viewed products

  •  
  • Product image

    Anti-Uroplakin III antibody [SFI-1], prediluted (ab78197)

  •  
  • Product image

    Anti-ADAMTSL1 antibody - N-terminal (ab155597)

  •  
  • Product image

    Anti-GluN2C antibody [EPR19046] - BSA and Azide free (ab251151)

  •  
  • Product image

    Anti-Keratin 12/K12 antibody [EPR1609(2)] - BSA and Azide free (ab248082)

  •  
  • Product image

    HRP Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] (ab196482)

  •  
  • Product image

    Anti-CYP3A7 antibody [EPR10650(2)(B)] - BSA and Azide free (ab249511)

Get resources and offers direct to your inbox Sign up
© 2021 Astana Biomed Group LLP. All rights reserved.