Lane 1: Wild-type HCT116 cell lysate (20µg)

Lane 2: SLC9A3R1 knockout HCT116 cell lysate (20µg)
Lanes 1- 2: Merged signal (red and green). Green - ab109430 observed at 48 kDa. Red - loading control ab8245 observed at 37 kDa.
ab109430 Anti-EBP50/NHERF-1 antibody [EPR5562] was shown to specifically react with EBP50/NHERF-1 in wild-type HCT116 cells in western blot. Loss of signal was observed when knockout cell line ab266876 (knockout cell lysate ab257281) was used. Wild-type and EBP50/NHERF-1 knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab109430 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4^°C at 1 in 1000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

Homozygous: 1 bp deletion in exon1