Human Siglec 9 ELISA Kit (CD329) (ab113339)
Key features and details
- Sensitivity: 130 pg/ml
- Range: 0.123 ng/ml - 30 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Siglec 9 ELISA Kit (CD329) -
Detection method
Colorimetric -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
Range
0.123 ng/ml - 30 ng/mlRecovery
85 %
Sample specific recovery Sample type Average % Range Cell culture supernatant 95.43 88% - 102% Serum 82.7 72% - 91% Plasma 78.16 70% - 91% Assay duration
Multiple steps standard assaySpecies reactivity
Reacts with: HumanProduct overview
Abcam’s Siglec 9 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Siglec 9 in serum, plasma, cell culture supernatants.
This assay employs an antibody specific for Human Siglec 9 coated on a 96-well plate. Standards and samples are pipetted into the wells and Siglec 9 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Siglec 9 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Siglec 9 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Notes
Optimization may be required with urine samples.
Platform
MicroplateProperties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent B 1 x 15ml 500X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent D 1 x 15ml Biotinylated anti-Human Siglec 9 2 vials Recombinant Human Siglec 9 Standard (lyophilized) 2 vials Siglec 9 Microplate (12 x 8 wells) 1 unit Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Database links
- SwissProt: Q9Y336 Human
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent B 1 x 15ml 500X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent D 1 x 15ml Biotinylated anti-Human Siglec 9 2 vials Recombinant Human Siglec 9 Standard (lyophilized) 2 vials Siglec 9 Microplate (12 x 8 wells) 1 unit Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
-
Database links
- SwissProt: Q9Y336 Human
Images
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Representative Standard Curve
Representative Standard Curve using ab113339
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Representative Standard CurveRepresentative Standard Curve using ab113339