Human SHBG ELISA Kit (ab260070)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 26 pg/ml
- Range: 62.5 pg/ml - 4000 pg/ml
- Sample type: Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human SHBG ELISA Kit
See all SHBG kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 3.8% Inter-assay Sample n Mean SD CV% Serum 3 4.6% -
Sample type
Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
26 pg/ml -
Range
62.5 pg/ml - 4000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 111 107% - 116% Hep Plasma 102 87% - 114% EDTA Plasma 107 94% - 115% Cit plasma 114 106% - 119% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human SHBG ELISA Kit (ab260070) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of SHBG protein in cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human SHBG with 26 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
SHBG, Sex hormone-binding globulin, is a steroid binding protein used to transport dihydrotestosterone, testosterone, and 17-beta-estradiol. It is crucial in the maintenance of plasma steroid hormone levels. This kit targets the mature, full-length, protein form which lacks the N-terminal signaling peptide.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human SHBG Capture Antibody 1 x 600µl 10X Human SHBG Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BI 1 x 6ml Human SHBG Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Functions as an androgen transport protein, but may also be involved in receptor mediated processes. Each dimer binds one molecule of steroid. Specific for 5-alpha-dihydrotestosterone, testosterone, and 17-beta-estradiol. Regulates the plasma metabolic clearance rate of steroid hormones by controlling their plasma concentration. -
Tissue specificity
Isoform 1 and isoform 2 are present in liver and testis. -
Sequence similarities
Contains 2 laminin G-like domains. -
Cellular localization
Secreted. In testis, it is synthesized by the Sertoli cells, secreted into the lumen of the seminiferous tubule and transported to the epididymis. - Information by UniProt
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Alternative names
- ABP
- Androgen binding protein
- SBP
see all -
Database links
- Entrez Gene: 6462 Human
- Omim: 182205 Human
- SwissProt: P04278 Human
- Unigene: 632235 Human
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human SHBG standard curve in Sample Diluent NS.The SHBG standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native SHBG in human serum and plasma samples.Interpolated concentrations of native SHBG in human serum and plasma samples. Samples were pooled from healthy 20 male and 20 female volunteers. The concentrations of SHBG were measured in duplicates, interpolated from the SHBG standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 0.2%, plasma (citrate) 0.2%, plasma (heparin) 0.2% and plasma (EDTA) 0.2%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean SHBG concentration was determined to be 1466 ng/mL in serum, 1453 ng/mL in plasma (Citrate),1559 ng/mL in plasma (Heparin), and 1348 ng/mL in plasma (EDTA).
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Sandwich ELISA - Human SHBG ELISA Kit (ab260070)To learn more about the advantages of recombinant antibodies see here.
