Human SFRP1 ELISA Kit (ab277468)
Key features and details
- Sensitivity: 0.78 ng/ml
- Range: 0.781 ng/ml - 50 ng/ml
- Sample type: Cell culture supernatant, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human SFRP1 ELISA Kit
See all SFRP1 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.78 ng/ml -
Range
0.781 ng/ml - 50 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 104 77% - 133% Plasma 102.4 79% - 114% Cell culture media 123 115% - 133% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human SFRP1 ELISA Kit (ab277468) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human SFRP1 in serum, plasma (collect plasma using EDTA and heparin as an anticoagulant. Citrate is not recommended as anticoagulants for use in this assay), and cell culture supernatants.
This assay employs an antibody specific for Human SFRP1 coated on a 96-well plate. Standards and samples are pipetted into the wells and Human SFRP1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human SFRP1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human SFRP1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent B 1 x 15ml Assay Diluent C 1 x 30ml Biotinylated Anti-Human SFRP1 Antibody 2 vials Anti-Human SFRP1 coated Microplate 1 unit Human SFRP1 standard protein (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP1 decreases intracellular beta-catenin levels (By similarity). Has antiproliferative effects on vascular cells, in vitro and in vivo, and can induce, in vivo, an angiogenic response. In vascular cell cycle, delays the G1 phase and entry into the S phase (By similarity). In kidney development, inhibits tubule formation and bud growth in metanephroi (By similarity). Inhibits WNT1/WNT4-mediated TCF-dependent transcription. -
Tissue specificity
Widely expressed. Absent from lung, liver and peripheral blood leukocytes. Highest levels in heart and fetal kidney. Also expressed in testis, ovary, fetal brain and lung, leiomyomal cells, myometrial cells and vascular smooth muscle cells. Expressed in foreskin fibroblasts and in keratinocytes. -
Sequence similarities
Belongs to the secreted frizzled-related protein (sFRP) family.
Contains 1 FZ (frizzled) domain.
Contains 1 NTR domain. -
Domain
The FZ domain is involved in binding with Wnt ligands. -
Cellular localization
Secreted. Cell membrane or extracellular matrix-associated. Released by heparin-binding. - Information by UniProt
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Alternative names
- Frizzled related protein 1
- FRP
- FRP 1
see all -
Database links
- Entrez Gene: 6422 Human
- Omim: 604156 Human
- SwissProt: Q8N474 Human
- Unigene: 213424 Human
Images
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Example data
These standard curves are for demonstration only. A standard curve must be run with each assay.