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Human SEMG2 ELISA Kit (ab240682)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 8.4 ng/ml
  • Range: 9.38 ng/ml - 600 ng/ml
  • Sample type: Serum, Urine
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

  • Product name

    Human SEMG2 ELISA Kit
    See all Semenogelin II/SGII kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    urine 8 2.2%

  • Sample type

    Urine, Serum

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    8.4 ng/ml

  • Range

    9.38 ng/ml - 600 ng/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Urine 81 % - %
    Serum 96 % - %

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    Human SEMG2 ELISA Kit (ab240682) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of SEMG2 protein in serum and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human SEMG2 with 8.4 ng/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Human SEMG2 Capture Antibody 1 x 600µl
    10X Human SEMG2 Detector Antibody 2 vials
    10X Wash Buffer PT (ab206977) 1 x 20ml
    Antibody Diluent 4BI 1 x 6ml
    Human SEMG2 Lyophilized recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml

  • Research areas

  • Function

    Participates in the formation of a gel matrix (sperm coagulum) entrapping the accessory gland secretions and ejaculated spermatozoa.

  • Tissue specificity

    Seminal vesicles, and to a much lesser extent, epididymis.

  • Sequence similarities

    Belongs to the semenogelin family.

  • Post-translational
    modifications

    Semenogelin-2 is thought to form both the 71 kDa polypeptide and, in its glycosylated form, the 76 kDa polypeptide.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Alternative names

    • Semenogelin 2
    • Semenogelin II
    • Semenogelin-2
    • SEMG2
    • SEMG2_HUMAN
    • SGII
    see all

  • Database links

    Images

    • Other - Human SEMG2 ELISA Kit (ab240682)
      Other - Human SEMG2 ELISA Kit (ab240682)

      SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

       

    • Example of human SEMG2 standard curve in Sample Diluent NS.
      Example of human SEMG2 standard curve in Sample Diluent NS.
      The SEMG2 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
    • Interpolated concentrations of native SEMG2 in human male urine and female urine samples.
      Interpolated concentrations of native SEMG2 in human male urine and female urine samples.

      The concentrations of SEMG2 were measured in duplicates, interpolated from the SEMG2 standard curves and corrected for sample dilution. Undiluted samples are as follows: male urine 1:17 and female urine 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean SEMG2 concentration was determined to be 79.2 µg/mL in male urine and was undetectable in female urine.

    • Interpolated concentrations of spiked SEMG2 in human serum and female urine samples.
      Interpolated concentrations of spiked SEMG2 in human serum and female urine samples.
      The concentrations of SEMG2 were measured in duplicates, interpolated from the SEMG2 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, and female urine 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
    • Sandwich ELISA - Human SEMG2 ELISA Kit (ab240682)
      Sandwich ELISA - Human SEMG2 ELISA Kit (ab240682)
      To learn more about the advantages of recombinant antibodies see here.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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