Human Semaphorin 7A ELISA Kit (ab272193)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 24.7 pg/ml
- Range: 70.31 pg/ml - 4500 pg/ml
- Sample type: Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Semaphorin 7A ELISA Kit
See all Semaphorin 7a kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 2.52% Inter-assay Sample n Mean SD CV% Serum 3 6.62% -
Sample type
Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
24.7 pg/ml -
Range
70.31 pg/ml - 4500 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 107 104% - 112% Hep Plasma 103 101% - 105% EDTA Plasma 104 103% - 105% Cit plasma 117 116% - 119% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human Semaphorin 7A ELISA kit (ab272193) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Semaphorin 7A protein in serum and plasma samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Semaphorin 7A with 24.7 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Semaphorins constitute a large class of soluble and membrane bound proteins that are known to be involved in critical immune responses and serve neuronal functions. Semaphorin 7A, also known as CD108, is a glycosylphosphatidylinositol-anchored semaphorin that promotes axon outgrowth through beta1-integrin receptors and contributes to the formation of the lateral olfactory tract. Promotes attachment, spreading and dendrite outgrowth in melanocytes and plays an important role in modulating inflammation and T-cell mediated immune response. Mouse, rat. cow and monkey share 89, 89%, 86% and 99% sequence homology, respectively.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human Semaphorin 7A Capture Antibody 1 x 600µl 10X Human Semaphorin 7A Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BI 1 x 6ml Human Semaphorin 7A Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Plays an important role in integrin-mediated signaling and functions both in regulating cell migration and immune responses. Promotes formation of focal adhesion complexes, activation of the protein kinase PTK2/FAK1 and subsequent phosphorylation of MAPK1 and MAPK3. Promotes production of proinflammatory cytokines by monocytes and macrophages. Plays an important role in modulating inflammation and T-cell-mediated immune responses. Promotes axon growth in the embryonic olfactory bulb. Promotes attachment, spreading and dendrite outgrowth in melanocytes. -
Tissue specificity
Detected in skin keratinocytes and on endothelial cells from skin blood vessels (at protein level). Expressed in fibroblasts, keratinocytes, melanocytes, placenta, testis, ovary, spleen, brain, spinal chord, lung, heart, adrenal gland, lymph nodes, thymus, intestine and kidney. -
Sequence similarities
Belongs to the semaphorin family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 PSI domain.
Contains 1 Sema domain. -
Cellular localization
Cell membrane. Detected in a punctate pattern on the cell membrane of basal and supra-basal skin keratinocytes. - Information by UniProt
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Alternative names
- CD 108
- CD108
- CD108 antigen
see all -
Database links
- Entrez Gene: 8482 Human
- Omim: 607961 Human
- SwissProt: O75326 Human
- Unigene: 24640 Human
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human Semaphorin 7A standard curve in Sample Diluent NS.The Semaphorin 7A standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Semaphorin 7A in human serum and plasma samples.The concentrations of Semaphorin 7A were measured in duplicate, interpolated from the Semaphorin 7A standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 10%, plasma (citrate) 10%, plasma (EDTA) 10%, and plasma (heparin) 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Semaphorin 7A concentration was determined to be 14,101 pg/mL in serum, 11,909 pg/mL in plasma (citrate) and 14,381 pg/mL in plasma (EDTA), and 10,604 pg/ml in plasma (heparin).
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Sandwich ELISA - Human Semaphorin 7A ELISA Kit (ab272193)To learn more about the advantages of recombinant antibodies see here.
