Human SCD (SCD1) knockout HeLa cell pellet (ab278976)
Properties
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Storage instructions
Store at -80°C. Please refer to protocols. -
Components 1 kit Human SCD knockout HeLa cell pellet 1 vial Human wild-type HeLa cell pellet 1 vial -
Research areas
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Cell type
epithelial -
Disease
Adenocarcinoma -
Gender
Female -
STR Analysis
Amelogenin X D5S818: 11, 12 D13S317: 12, 13.3 D7S820: 8, 12 D16S539: 9, 10 vWA: 16, 18 TH01: 7 TPOX: 8,12 CSF1PO: 9, 10
Images
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Western blot - Human SCD (SCD1) knockout HeLa cell pellet (ab278976)
Lane 1: Wild-type HeLa cell lysate 20 ug
Lane 2: SCD knockout HeLa cell lysate 20 ug
Lane 3: HepG2 cell lysate 20 ug
Lanes 1 - 3:Merged signal (red and green). Green - ab19862 observed at 36 kDa. Red - loading control, ab2866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) observed at 55kDa.
ab19862 was shown to react with SCD1 in wild-type HeLa cells in western blot with loss of signal observed in SCD knockout cell line ab262177 (SCD knockout cell lysate ab257658). HeLa wild-type and SCD knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab19862 and ab2866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) overnight at 4°C at a 1 in 1000 Dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging. -
Western blot - Human SCD (SCD1) knockout HeLa cell pellet (ab278976)Lane 1: Wild-type HeLa cell lysate (20 ug)
Lane 2: SCD knockout HeLa cell lysate (20 ug)
Lane 3: HepG2 cell lysate (20 ug)
Lanes 1-3: Merged signal (red and green). Green - ab236868. Red - loading control ab8245 observed at 50 kDa.
ab236868 Anti-SCD1 antibody [EPR21963] was shown to specifically react with SCD1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265220 (knockout cell lysate ab257658) was used. Wild-type and SCD1 knockout samples were subjected to SDS-PAGE. ab236868 and Anti-tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
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Sanger Sequencing - Human SCD (SCD1) knockout HeLa cell pellet (ab278976)Allele-1: 4 bp deletion in exon3
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Sanger Sequencing - Human SCD (SCD1) knockout HeLa cell pellet (ab278976)Allele-2: 4 bp deletion in exon3
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Sanger Sequencing - Human SCD (SCD1) knockout HeLa cell pellet (ab278976)Allele-3: 4 bp deletion in exon3
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Sanger Sequencing - Human SCD (SCD1) knockout HeLa cell pellet (ab278976)Allele-4: Insertion of the selection cassette in exon3
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Sanger Sequencing - Human SCD (SCD1) knockout HeLa cell pellet (ab278976)Allele-5: Insertion of the selection cassette in exon3