Human Retinol binding protein ELISA Kit (RBP) (ab108899)
Key features and details
- Sensitivity: 0.0078 µg/ml
- Range: 0.008 µg/ml - 0.5 µg/ml
- Sample type: Cell culture supernatant, Milk, Saliva, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Retinol binding protein ELISA Kit (RBP)
See all Retinol binding protein kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 4.6% Inter-assay Sample n Mean SD CV% Overall 7.2% -
Sample type
Cell culture supernatant, Saliva, Milk, Urine -
Assay type
Sandwich (quantitative) -
Sensitivity
0.0078 µg/ml -
Range
0.008 µg/ml - 0.5 µg/ml -
Recovery
98 %
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Assay time
4h 00m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s Retinol binding protein (RBP) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Retinol binding protein concentrations in Human milk, urine, CSF, saliva and cell culture supernatants.
A Retinol binding protein specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Retinol binding protein specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Retinol binding protein captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 40X Biotinylated Human RBP Antibody 1 x 150µl Chromogen Substrate 1 x 8ml Retinol binding protein Microplate (12 x 8 well strips) 1 unit Retinol binding protein Standard 1 vial Sealing Tapes 3 units Stop Solution 1 x 12ml -
Research areas
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Database links
- SwissProt: P09455 Human
- SwissProt: P50120 Human
- SwissProt: P10745 Human
- SwissProt: P02753 Human
- Unigene: 5950 Human
Images
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Sandwich ELISA - ab108899 Retinol binding protein (RBP) Human ELISA Kit
RBP measured in various samples showing quantity (pg) per mL of tested sample
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Typical Standard CurveRepresentative Standard Curve using ab108899.
