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Signal Transduction Signaling Pathway G Protein Signaling Small G Proteins Ras Family

Human RASGRP3 knockout A549 cell lysate (ab259078)

Price and availability

318 288 ₸

Availability

Order now and get it on Friday March 05, 2021

Human RASGRP3 knockout A549 cell lysate (ab259078)
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Overview

  • Product name

    Human RASGRP3 knockout A549 cell lysate
    See all RASGRP3 kits
  • Product overview


    Knockout cell lysate achieved by CRISPR/Cas9.

  • Parental Cell Line

    A549
  • Organism

    Human
  • Mutation description

    Knockout achieved by using CRISPR/Cas9, 2 bp deletion in exon8.
  • Passage number

  • Knockout validation

    Sanger Sequencing
  • Reconstitution notes

    To use as WB control, resuspend the lyophilizate in 50 µL of LDS* Sample Buffer to have a final concentration of 2 mg/ml. For reducing conditions, we recommend a final concentration of 0.1 M DTT.

    *Usage of SDS sample buffer is not recommended with these lyophilized lysates.

  • Notes

    Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version - found here. Please refer to our lysis protocol for further details on how our lysates are prepared.

    User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

    Access thousands of knockout cell lysates, generated from commonly used cancer cell lines.
    See here for more information on knockout cell lysates.

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

    This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

Properties

  • Storage instructions

    Store at -80°C. Please refer to protocols.
  • Components 1 kit
    ab262615 - Human RASGRP3 knockout A549 cell lysate 1 x 100µg
    ab255554 - Human wild-type A549 cell lysate 1 x 100µg
  • Research areas

    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • Small G Proteins
    • Ras Family
    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • GPCR
  • Cell type

    epithelial
  • Disease

    Carcinoma
  • STR Analysis

    Amelogenin X,Y D5S818: 11 D13S317: 11 D7S820: 8, 11 D16S539: 11, 12 vWA: 14 TH01: 8,9.3 TPOX: 8,11 CSF1PO: 10, 12

Target

  • Function

    Guanine nucleotide exchange factor (GEF) for Ras and Rap1.
  • Sequence similarities

    Belongs to the RASGRP family.
    Contains 2 EF-hand domains.
    Contains 1 N-terminal Ras-GEF domain.
    Contains 1 phorbol-ester/DAG-type zinc finger.
    Contains 1 Ras-GEF domain.
  • Target information above from: UniProt accession Q8IV61 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • Calcium and DAG-regulated guanine nucleotide exchange factor III
    • Grp3
    • GRP3_HUMAN
    • Guanine nucleotide exchange factor for Rap1
    • Kiaa0846
    • RAS guanyl releasing protein 3 (calcium and DAG regulated)
    • RAS guanyl releasing protein 3 calcium and DAG regualated guanine nucleotide exchange factor iii
    • Ras guanyl-releasing protein 3
    • RASGRP3
    see all

Properties

  • Storage instructions

    Store at -80°C. Please refer to protocols.
  • Components 1 kit
    ab262615 - Human RASGRP3 knockout A549 cell lysate 1 x 100µg
    ab255554 - Human wild-type A549 cell lysate 1 x 100µg
  • Research areas

    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • Small G Proteins
    • Ras Family
    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • GPCR
  • Cell type

    epithelial
  • Disease

    Carcinoma
  • STR Analysis

    Amelogenin X,Y D5S818: 11 D13S317: 11 D7S820: 8, 11 D16S539: 11, 12 vWA: 14 TH01: 8,9.3 TPOX: 8,11 CSF1PO: 10, 12

Images

  • Sanger Sequencing - Human RASGRP3 knockout A549 cell lysate (ab259078)
    Sanger Sequencing - Human RASGRP3 knockout A549 cell lysate (ab259078)
    Homozygous: 2 bp deletion in exon8

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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