Human PTTG1 (Securin) knockout HEK293T cell pellet (ab279028)
Properties
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Storage instructions
Store at -80°C. Please refer to protocols. -
Components 1 kit Human PTTG1 knockout HEK293T cell pellet 1 vial Human wild-type HEK293T cell pellet 1 vial -
Research areas
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Cell type
epithelial -
STR Analysis
Amelogenin X D5S818: 8, 9 D13S317: 12, 14 D7S820: 11 D16S539: 9, 13 vWA: 16, 19 TH01: 7, 9.3 TPOX: 11 CSF1PO: 11, 12
Images
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Western blot - Human PTTG1 (Securin) knockout HEK293T cell pellet (ab279028)
Lane 1: Wild-type HEK293T cell lysate (20 µg)
Lane 2: PTTG1 knockout HEK293T cell lysate (20 µg)
Lane 3: Daudi cell lysate (20 µg)
Lanes 1-3: Merged signal (red and green). Green - ab79546 observed at 28 kDa. Red - loading control, ab8245 observed at 37 kDa.
ab79546 Anti-Securin antibody [EPR3240] was shown to specifically react with Securin in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266231 (knockout cell lysate ab257289) was used. Wild-type and Securin knockout samples were subjected to SDS-PAGE. ab79546 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
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Western blot - Human PTTG1 (Securin) knockout HEK293T cell pellet (ab279028)Lane 1: Wild-type HEK293T cell lysate (20 µg)
Lane 2: PTTG1 knockout HEK293T cell lysate (20 µg)
Lane 3: Daudi cell lysate (20 µg)
Lanes 1-3: Merged signal (red and green). Green - ab26273 observed at 28 kDa. Red - loading control, ab8245 observed at 37 kDa.
ab26273 Anti-Securin antibody was shown to specifically react with Securin in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266231 (knockout cell lysate ab257289) was used. Wild-type and Securin knockout samples were subjected to SDS-PAGE. ab26273 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
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Sanger Sequencing - Human PTTG1 (Securin) knockout HEK293T cell pellet (ab279028)Homozygous: 8 bp deletion in exon 1
