Human PTH ELISA Kit (ab230931)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 0.761 pg/ml
- Range: 4.69 pg/ml - 300 pg/ml
- Sample type: Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
-
Product name
Human PTH ELISA Kit
See all Parathyroid Hormone kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Spiked serum 8 1.5% Inter-assay Sample n Mean SD CV% Spiked serum 3 3.8% -
Sample type
Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.761 pg/ml -
Range
4.69 pg/ml - 300 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 88 88% - 89% Cell culture media 105 104% - 106% Hep Plasma 83 81% - 84% EDTA Plasma 90 88% - 92% Cit plasma 82 81% - 82% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human PTH ELISA Kit (ab230931) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of PTH protein in cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human PTH with 0.761 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
-
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
-
Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human PTH Capture Antibody 1 x 600µl 10X Human PTH Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BI 1 x 6ml Human PTH Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
-
Function
PTH elevates calcium level by dissolving the salts in bone and preventing their renal excretion. Stimulates [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblastic cells. -
Involvement in disease
Defects in PTH are a cause of familial isolated hypoparathyroidism (FIH) [MIM:146200]; also called autosomal dominant hypoparathyroidism or autosomal dominant hypocalcemia. FIH is characterized by hypocalcemia and hyperphosphatemia due to inadequate secretion of parathyroid hormone. Symptoms are seizures, tetany and cramps. FIH exist both as autosomal dominant and recessive forms of hypoparathyroidism. -
Sequence similarities
Belongs to the parathyroid hormone family. -
Cellular localization
Secreted. - Information by UniProt
-
Alternative names
- hPTH
- Parathormone
- Parathyrin
see all -
Database links
- Entrez Gene: 5741 Human
- Omim: 168450 Human
- SwissProt: P01270 Human
- Unigene: 37045 Human
Images
-
Other - Human PTH ELISA Kit (ab230931)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
-
Example of Human PTH standard curve in Sample Diluent NS.Background-subtracted data values (mean +/- SD) are graphed.
-
Interpolated concentrations of spiked PTH in Human serum, plasma and cell culture media samples.The concentrations of PTH were measured in duplicate, interpolated from the PTH standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, plasma (EDTA) 50%, plasma (heparin) 50% and 10F RPMI media 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PTH concentration was determined to be 262.33 pg/mL in serum, 258.51 pg/mL in plasma (citrate) and 264.22 pg/mL in plasma (EDTA), 249.32 pg/mL in plasma (heparin), and 300.25 pg/mL in 10F RPMI media 100%.
-
Sandwich ELISA - Human PTH ELISA Kit (ab230931)To learn more about the advantages of recombinant antibodies see here.
