Human PTBP1 knockout HeLa cell pellet (ab279036)
Overview
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Product name
Human PTBP1 knockout HeLa cell pellet
See all PTBP1 kits -
Product overview
Abcam’s knockout cell pellets give you access to native proteins, without the need to culture cells. Our knockout cell pellets are prepared from our single-gene knockout cell lines and provide an additional offering to our cell lysates.
Cells are snap-frozen to provide high quality pellets that are suitable for extraction with alternative lysis buffers or for preparation of lysates from subcellular fractions. Our knockout cell pellets are suitable for a variety of applications, including PCR, gene expression profiling and DNA library preparation. -
Parental Cell Line
HeLa -
Organism
Human -
Mutation description
Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon4. -
Passage number
Knockout validation
Sanger Sequencing, Western Blot (WB)Notes
Pellet size: 5 million cells/vial.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
Tested applications
Suitable for: WBmore detailsProperties
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Storage instructions
Store at -80°C. Please refer to protocols. -
Components 1 kit Human PTBP1 knockout HeLa cell pellet 1 vial Human wild-type HeLa cell pellet 1 vial -
Research areas
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Cell type
epithelial -
Disease
Adenocarcinoma -
Gender
Female -
STR Analysis
Amelogenin X D5S818: 11, 12 D13S317: 12, 13.3 D7S820: 8, 12 D16S539: 9, 10 vWA: 16, 18 TH01: 7 TPOX: 8,12 CSF1PO: 9, 10
Target
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Function
Plays a role in pre-mRNA splicing and in the regulation of alternative splicing events. Binds to the polypyrimidine tract of introns. May promote RNA looping when bound to two separate polypyrimidine tracts in the same pre-mRNA. May promote the binding of U2 snRNP to pre-mRNA. Cooperates with RAVER1 to modulate switching between mutually exclusive exons during maturation of the TPM1 pre-mRNA. -
Sequence similarities
Contains 4 RRM (RNA recognition motif) domains. -
Cellular localization
Nucleus. - Information by UniProt
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Alternative names
- 57 kDa RNA binding protein PPTB 1
- 57 kDa RNA-binding protein PPTB-1
- Heterogeneous nuclear ribonucleoprotein I
see all
Properties
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Storage instructions
Store at -80°C. Please refer to protocols. -
Components 1 kit Human PTBP1 knockout HeLa cell pellet 1 vial Human wild-type HeLa cell pellet 1 vial -
Research areas
-
Cell type
epithelial -
Disease
Adenocarcinoma -
Gender
Female -
STR Analysis
Amelogenin X D5S818: 11, 12 D13S317: 12, 13.3 D7S820: 8, 12 D16S539: 9, 10 vWA: 16, 18 TH01: 7 TPOX: 8,12 CSF1PO: 9, 10
Images
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Western blot - Human PTBP1 knockout HeLa cell pellet (ab279036)Lane 1: Wild-type HeLa cell lysate (20µg)
Lane 2: PTBP1 knockout HeLa cell lysate (20µg)
Lanes 1- 2: Merged signal (red and green). Green - ab134950 observed at 57 kDa. Red - loading control ab8245 observed at 37 kDa.
ab134950 Anti-PTBP1 antibody [EPR9049(B)] was shown to specifically react with PTBP1 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab265155 (knockout cell lysate ab257614) was used. Wild-type and PTBP1 knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab134950 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging. -
Western blot - Human PTBP1 knockout HeLa cell pellet (ab279036)Lane 1: Wild-type HeLa cell lysate (20µg)
Lane 2: PTBP1 knockout HeLa cell lysate (20µg)
Lanes 1- 2: Merged signal (red and green). Green - ab133734 observed at 57 kDa. Red - loading control ab8245 observed at 37 kDa.
ab133734 Anti-PTBP1 antibody [EPR9048(B)] was shown to specifically react with PTBP1 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab265155 (knockout cell lysate ab257614) was used. Wild-type and PTBP1 knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab133734 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 10000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging. -
Sanger Sequencing - Human PTBP1 knockout HeLa cell pellet (ab279036)Homozygous: 1 bp insertion in exon4