Human PSMB8 knockout A549 cell pellet (ab279223)
Properties
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Storage instructions
Store at -80°C. Please refer to protocols. -
Components 1 kit Human PSMB8 knockout A549 cell pellet 1 vial Human wild-type A549 cell pellet 1 vial -
Research areas
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Cell type
epithelial -
Disease
Carcinoma -
STR Analysis
Amelogenin X,Y D5S818: 11 D13S317: 11 D7S820: 8, 11 D16S539: 11, 12 vWA: 14 TH01: 8,9.3 TPOX: 8,11 CSF1PO: 10, 12
Images
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Western blot - Human PSMB8 knockout A549 cell pellet (ab279223)
Lane 1:Wild-type A549 (Human lung carcinoma cell line) whole cell lysate (20 ug)
Lane 2:PSMB8 knockout A549 (Human lung carcinoma cell line) whole cell lysate (20 ug)
Lane 3:Raji (Human Burkitt's lymphoma cell line) whole cell lysate (20 ug)
Lane 4:HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (20 ug)ab180606 was shown to specifically react with Proteasome 20S LMP7 in wild-type A549 cells. Loss of signal was observed when knockout cell line ab267149 (knockout cell lysate ab257130) was used. Wild-type and Proteasome 20S LMP7 knockout samples were subjected to SDS-PAGE. ab180606 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Western blot - Human PSMB8 knockout A549 cell pellet (ab279223)Lane 1:Wild-type A549 (Human lung carcinoma cell line) whole cell lysate (20 ug)
Lane 2:PSMB8 knockout A549 (Human lung carcinoma cell line) whole cell lysate (20 ug)
Lane 3:Raji (Human Burkitt's lymphoma cell line) whole cell lysate (20 ug)
Lane 4:HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (20 ug)ab82528 was shown to specifically react with Proteasome 20S LMP7 in wild-type A549 cells. Loss of signal was observed when knockout cell line ab267149 (knockout cell lysate ab257130) was used. Wild-type and Proteasome 20S LMP7 knockout samples were subjected to SDS-PAGE. ab82528 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4oC at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Sanger Sequencing - Human PSMB8 knockout A549 cell pellet (ab279223)Allele-1: 1 bp deletion in exon3
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Sanger Sequencing - Human PSMB8 knockout A549 cell pellet (ab279223)Allele-2: 1 bp insertion in exon3