Anti-PSMD9 antibody [EPR12947] - BSA and Azide free (ab250563)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12947] to PSMD9 - BSA and Azide free
- Suitable for: IHC-P, ICC, WB, Flow Cyt (Intra)
- Reacts with: Human
Overview
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Product name
Anti-PSMD9 antibody [EPR12947] - BSA and Azide free
See all PSMD9 primary antibodies -
Description
Rabbit monoclonal [EPR12947] to PSMD9 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC, WB, Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab250563 is the carrier-free version of ab181998.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR12947 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-PSMD9 antibody [EPR12947] (ab181998) at 1/1000 dilution
Lane 1 : Human fetal liver tissue lysate
Lane 2 : HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : 293 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), HRP at 1/1000 dilution
Predicted band size: 25 kDaThis data was developed using ab181998, the same antibody clone in a different buffer formulation.
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This data was developed using ab181998, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling PSMD9 with ab181998 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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This data was developed using ab181998, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of HeLa cells (-20°C acetone-fixed) labeling PSMD9 with ab181998 at 1/500 dilution followed by Goat anti rabbit IgG (Dylight 555) secondary and counter-stained with DAPI (blue).
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This data was developed using ab181998, the same antibody clone in a different buffer formulation.Flow cytometric analysis of HeLa cells (paraformaldehyde-fixed, 2%) labeling PSMD9 with ab181998 at 1/300 dilution (red) or a rabbit IgG (negative) (green), followed by secondary Goat anti-rabbit IgG (FITC) at 1/150 dilution.
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This data was developed using ab181998, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of HepG2 cells (paraformaldehyde-fixed, 4%) labeling PSMD9 with ab181998 at 1/500 dilution. A Goat anti rabbit IgG(Alexa Fluor® 555) was used as secondary antibody. Counterstained with DAPI (blue).
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