Human Protein C ELISA Kit (ab137987)
Key features and details
- Sensitivity: 3 ng/ml
- Range: 3.13 ng/ml - 200 ng/ml
- Sample type: Cell culture supernatant, Milk, Saliva, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Protein C ELISA Kit
See all Protein C kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall = 4.8% Inter-assay Sample n Mean SD CV% Overall = 7.3% -
Sample type
Cell culture supernatant, Saliva, Milk, Urine -
Assay type
Sandwich (quantitative) -
Sensitivity
= 3 ng/ml -
Range
3.13 ng/ml - 200 ng/ml -
Recovery
= 98 %
Sample specific recovery Sample type Average % Range Saliva 94% - 99% Milk 94% - 98% Urine 89% - 93% -
Assay time
4h 0m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s Protein C Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Protein C in urine, saliva, milk, and cell culture supernatant.
A Protein C specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Protein C specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Protein C captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated Human Protein C Antibody 1 x 120µl Chromogen Substrate 1 x 8ml Protein C Microplate (12 x 8 well strips) 1 unit Protein C Standard 1 vial Sealing Tapes 3 units Stop Solution 1 x 12ml -
Research areas
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Function
Protein C is a vitamin K-dependent serine protease that regulates blood coagulation by inactivating factors Va and VIIIa in the presence of calcium ions and phospholipids (PubMed:25618265). Exerts a protective effect on the endothelial cell barrier function (PubMed:25651845). -
Tissue specificity
Plasma; synthesized in the liver. -
Involvement in disease
Thrombophilia due to protein C deficiency, autosomal dominant
Thrombophilia due to protein C deficiency, autosomal recessive -
Sequence similarities
Belongs to the peptidase S1 family.
Contains 2 EGF-like domains.
Contains 1 Gla (gamma-carboxy-glutamate) domain.
Contains 1 peptidase S1 domain. -
Post-translational
modificationsThe vitamin K-dependent, enzymatic carboxylation of some Glu residues allows the modified protein to bind calcium.
N- and O-glycosylated. Partial (70%) N-glycosylation of Asn-371 with an atypical N-X-C site produces a higher molecular weight form referred to as alpha. The lower molecular weight form, not N-glycosylated at Asn-371, is beta. O-glycosylated with core 1 or possibly core 8 glycans.
The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.
May be phosphorylated on a Ser or Thr in a region (AA 25-30) of the propeptide. -
Cellular localization
Secreted. Golgi apparatus. Endoplasmic reticulum. - Information by UniProt
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Alternative names
- Activation peptide
- Anticoagulant protein C
- APC
see all -
Database links
- Entrez Gene: 5624 Human
- Omim: 612283 Human
- SwissProt: P04070 Human
- Unigene: 224698 Human
Images
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Typical Standard Curve
Representative Standard Curve using ab137987
