Human Prorenin ELISA Kit (ab169738)
Key features and details
- Sensitivity: 0.016 ng/ml
- Range: 0.02 ng/ml - 10 ng/ml
- Sample type: Cell culture media, Tissue Extracts, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Prorenin ELISA Kit
See all Prorenin kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% 1 20 0.126ng/ml 0.009 7.28% 2 20 0.523ng/ml 0.028 4.88% 3 20 5.12ng/ml 0.243 4.74% Inter-assay Sample n Mean SD CV% 1 10 0.165ng/ml 0.016 9.7% 2 10 0.532ng/ml 0.031 5.85% 3 10 5.03ng/ml 0.203 4.04% -
Sample type
Urine, Tissue Extracts, Cell culture media -
Assay type
Sandwich (quantitative) -
Sensitivity
0.016 ng/ml -
Range
0.02 ng/ml - 10 ng/ml -
Recovery
108.75 %
Sample specific recovery Sample type Average % Range Cell culture media 108.75 98% - 132% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s Prorenin ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative determination of Prorenin in non-plasma samples including cell culture media, tissue extracts, or urine samples. Active renin will not be detected by this assay. Prorenin is measured directly by ELISA without pretreatment of samples or conversion to renin.
Human Prorenin will bind to the capture antibody coated on the microtiter plate. After appropriate washing steps, anti-Human Prorenin primary antibody binds to the captured protein. Only Prorenin and not active renin will be detected by the primary antibody. Excess antibody is washed away and bound primary antibody is then reacted with the secondary antibody conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of Prorenin. The amount of color development is directly proportional to the concentration of Prorenin in the sample.
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Platform
Microplate
Properties
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Storage instructions
Please refer to protocols. -
Components 1 x 96 tests 10X Wash Buffer 1 x 50ml Anti-Human Prorenin antibody coated plates 1 unit Anti-Human Prorenin Primary Antibody 1 vial Anti-mouse HRP Secondary Antibody 1 vial Human Prorenin Standard 1 vial TMB Substrate Solution 1 x 10ml -
Research areas
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Function
Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney. -
Involvement in disease
Defects in REN are a cause of renal tubular dysgenesis (RTD) [MIM:267430]. RTD is an autosomal recessive severe disorder of renal tubular development characterized by persistent fetal anuria and perinatal death, probably due to pulmonary hypoplasia from early-onset oligohydramnios (the Potter phenotype).
Defects in REN are the cause of familial juvenile hyperuricemic nephropathy type 2 (HNFJ2) [MIM:613092]. It is a renal disease characterized by juvenile onset of hyperuricemia, slowly progressive renal failure and anemia. -
Sequence similarities
Belongs to the peptidase A1 family. -
Cellular localization
Secreted. Membrane. Associated to membranes via binding to ATP6AP2. - Information by UniProt
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Alternative names
- Angiotensin forming enzyme
- Angiotensinogenase
- FLJ10761
see all -
Database links
- Entrez Gene: 5972 Human
- Omim: 179820 Human
- SwissProt: P00797 Human
- Unigene: 3210 Human
Images
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Example Standard Curve for ab169738
