Human Pro-Collagen I C-Terminal Propeptide ELISA Kit (PICP) (ab272198)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 8.15 pg/ml
- Range: 23.44 pg/ml - 1500 pg/ml
- Sample type: Cell culture media, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Pro-Collagen I C-Terminal Propeptide ELISA Kit (PICP)
See all PICP kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 3.1% Inter-assay Sample n Mean SD CV% Serum 3 7.2% -
Sample type
Serum, Cell culture media, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
8.15 pg/ml -
Range
23.44 pg/ml - 1500 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 102 92% - 109% Cell culture media 107 103% - 109% Hep Plasma 89 85% - 92% EDTA Plasma 89 85% - 100% Cit plasma 90 80% - 106% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human Pro-Collagen I C-Terminal Propeptide ELISA kit (PICP) (ab272198) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Pro-Collagen I C-Terminal Propeptide protein in serum, plasma and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human PICP with 8.15 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Pro-Collagen I C-Terminal Propeptide (PICP), encoded by the COL1A1 gene, is the fibrillar collagen that serves as a major component of type I collagen found in cartilage and most connective tissues. Studies have shown that mutations in the mutations in the Pro-Collagen I C-Terminal Propeptide are associated with osteoporosis, osteogenesis imperfecta diseases, and Ehlers-Danlos syndrome. The standard protein in this product is the C-terminal Propeptide region of Collagen alpha-1(I).
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human Pro-Collagen I C-Terminal Propeptide (PICP) Capture Antibody 1 x 600µl 10X Human Pro-Collagen I C-Terminal Propeptide (PICP) Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml Human Pro-Collagen I C-Terminal Propeptide (PICP) Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Type I collagen is a member of group I collagen (fibrillar forming collagen). -
Tissue specificity
Forms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite. -
Involvement in disease
Caffey disease
Ehlers-Danlos syndrome 1
Ehlers-Danlos syndrome 7A
Osteogenesis imperfecta 1
Osteogenesis imperfecta 2
Osteogenesis imperfecta 3
Osteogenesis imperfecta 4
Osteoporosis
A chromosomal aberration involving COL1A1 is found in dermatofibrosarcoma protuberans. Translocation t(17;22)(q22;q13) with PDGF. -
Sequence similarities
Belongs to the fibrillar collagen family.
Contains 1 fibrillar collagen NC1 domain.
Contains 1 VWFC domain. -
Domain
The C-terminal propeptide, also known as COLFI domain, have crucial roles in tissue growth and repair by controlling both the intracellular assembly of procollagen molecules and the extracellular assembly of collagen fibrils. It binds a calcium ion which is essential for its function. -
Post-translational
modificationsProline residues at the third position of the tripeptide repeating unit (G-X-P) are hydroxylated in some or all of the chains. Proline residues at the second position of the tripeptide repeating unit (G-P-X) are hydroxylated in some of the chains.
O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Alternative names
- COL1A1
- Alpha 1 type I collagen
- Alpha-1 type I collagen
see all -
Database links
- Entrez Gene: 1277 Human
- Omim: 120150 Human
- SwissProt: P02452 Human
- Unigene: 172928 Human
- Unigene: 681002 Human
Images
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SimpleStep ELISA Protocol Diagram
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Example of human Pro-Collagen I C-Terminal Propeptide standard curve in Sample Diluent NS. -
Interpolated concentrations of native Pro-Collagen I C-Terminal Propeptide in human serum and plasma samples. -
Interpolated concentrations of native Pro-Collagen I C-Terminal Propeptide in human skin tissue extract sample based on a 50 µg/mL extract load. -
Serum from ten individual healthy human female donors was measured in duplicate. -
Sandwich ELISA - Human Pro-Collagen I C-Terminal Propeptide ELISA Kit (PICP) (ab272198)To learn more about the advantages of recombinant antibodies see here.
