Human PRKCD (PKC delta) knockout HEK-293T cell lysate (ab257042)
Properties
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Storage instructions
Store at -80°C. Please refer to protocols. -
Components 1 kit ab262820 - Human PRKCD knockout HEK293T cell lysate 1 x 100µg ab255553 - Human wild-type HEK293T cell lysate 1 x 100µg -
Research areas
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Cell type
epithelial -
STR Analysis
Amelogenin X D5S818: 8, 9 D13S317: 12, 14 D7S820: 11 D16S539: 9, 13 vWA: 16, 19 TH01: 7, 9.3 TPOX: 11 CSF1PO: 11, 12
Images
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Western blot - Human PRKCD (PKC delta) knockout HEK293T cell lysate (ab257042)
Lane 1: Wild-type HEK-293T cell lysate 20 ug
Lane 2: PRKCD knockout HEK-293T cell lysate 20 ug
Lanes 1 - 2: Merged signal (red and green). Green - ab182126 observed at 78 kDa. Red - loading control, ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.#ab182126 was shown to react with PKC in wild-type HeLa cells in western blot. The bands observed in PRKCD knockout cell line ab266143 (PRKCD knockout cell lysate ab257042) below 78kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type HeLa and PRKCD knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab182126 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 5000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging. -
Sanger Sequencing - Human PRKCD knockout HEK293T cell lysate (ab257042)Allele-1: 2 bp deletion in exon2
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Sanger Sequencing - Human PRKCD knockout HEK293T cell lysate (ab257042)Allele-2: 1 bp insertion in exon2