Human Plunc ELISA Kit (ab277427)
Key features and details
- Sensitivity: 40 pg/ml
- Range: 40.96 pg/ml - 10000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Plunc ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
40 pg/ml -
Range
40.96 pg/ml - 10000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 142.7 138% - 145% Plasma 128.4 76% - 147% Cell culture media 119.2 87% - 137% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human Plunc ELISA Kit (ab277427) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Plunc in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human Plunc coated on a 96-well plate. Standards and samples are pipetted into the wells and Human Plunc present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human Plunc antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human Plunc bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 550X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent 1 x 15ml Biotinylated Anti-Human Plunc Antibody 2 vials Anti-Human Plunc coated Microplate 1 unit Human Plunc standard protein (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Relevance
May be involved in the airway inflammatory response after exposure to irritants. May be associated with tumor progression. May play a role in innate immune responses of the upper airways. -
Cellular localization
Secreted. Note: Found in the nasal mucus. Apical side of airway epithelial cells. Detected in nasal mucus -
Alternative names
- BPIFA1
- bA49G10.5
- LPLUNC3
see all -
Database links
- Entrez Gene: 51297 Human
- Omim: 607412 Human
- SwissProt: Q9NP55 Human
Images
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Example data
These standard curves are for demonstration only. A standard curve must be run with each assay.