Astana Biomed Group, an authorized Abcam distributor in Central Asia

Human PF4 ELISA Kit (CXCL4) (ab189573)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 24.29 pg/ml
  • Range: 78.13 pg/ml - 5000 pg/ml
  • Sample type: Cell culture media, Cell culture supernatant, Cit plasma, EDTA Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

  • Product name

    Human PF4 ELISA Kit (CXCL4)
    See all PF4 kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 8 3.7%
    Inter-assay
    Sample n Mean SD CV%
    Overall 3 3.5%

  • Sample type

    Cell culture supernatant, Serum, Cell culture media, EDTA Plasma, Cit plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    24.29 pg/ml

  • Range

    78.13 pg/ml - 5000 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 108 100% - 115%
    Serum 93 89% - 99%
    Cell culture media 87 85% - 90%
    EDTA Plasma 103 97% - 115%
    Cit plasma 92 83% - 100%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    As of November 23, 2020, Human PF4 (CXCL4) ELISA kit has been re-developed. We have identified new recombinant monoclonal antibodies to provide improved performance and consistency.


    Human PF4 ELISA Kit (CXCL4) (ab189573) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of PF4 (CXCL4) protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human PF4 (CXCL4) with 24.29 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    Platelet factor-4 (PF4/CXCL4) is a 70-amino acid protein that is released from the alpha-granules of activated platelets whose major physiologic role appears to be inhibiting local antithrombin III activity and promoting coagulation. PF4 (CXCL4) has a role in inflammation and wound repair by acting as a strong chemoattractant for neutrophils, monocytes, and fibroblasts.

  • Platform

    Microplate

Properties

Images

  • Other - Human PF4 ELISA Kit (CXCL4) (ab189573)
    Other - Human PF4 ELISA Kit (CXCL4) (ab189573)

    SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Example of human PF4 standard curve in Sample Diluent NS.
    Example of human PF4 standard curve in Sample Diluent NS.

    Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

  • Interpolated concentrations of native PF4 in human serum, plasma and cell culture supernatant samples.
    Interpolated concentrations of native PF4 in human serum, plasma and cell culture supernatant samples.

    The concentrations of PF4 were measured in duplicates, interpolated from the PF4 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 2%, plasma (citrate) 0.5%, and plasma (EDTA) 4%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PF4 concentration was determined to be 249.53 ng/mL in serum, 622.09 ng/mL in plasma (citrate), and 117.28 ng/mL in plasma (EDTA).

  • Interpolated concentrations of native PF4 in human cell culture supernatant samples.
    Interpolated concentrations of native PF4 in human cell culture supernatant samples.

    The concentrations of PF4 were measured in duplicates, interpolated from the PF4 standard curves and corrected for sample dilution. Undiluted samples are as follows: PBMC cell culture supernatant 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PF4 concentration was determined to be 19.25 ng/mL in PBMC cell culture supernatant.

  • 0.5% Serum from ten individual healthy human female donors was measured in duplicate.
    0.5% Serum from ten individual healthy human female donors was measured in duplicate.

    Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PF4 concentration was determined to be 267.15 ng/mL with a range of 47.05 – 986.18 ng/mL.

  • 0.5% Serum from ten individual healthy human male donors was measured in duplicate.
    0.5% Serum from ten individual healthy human male donors was measured in duplicate.

    Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PF4 concentration was determined to be 104.38 ng/mL with a range of 27.73 – 353.63 ng/mL.

  • Sandwich ELISA - Human PF4 ELISA Kit (CXCL4) (ab189573)
    Sandwich ELISA - Human PF4 ELISA Kit (CXCL4) (ab189573)
    To learn more about the advantages of recombinant antibodies see here.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Human PF4 ELISA Kit (CXCL4) (ab189573)

© 2021 Astana Biomed Group LLP. All rights reserved.